The effect of TGF-alpha and EGF to the liver regenerat

用四氯化碳和苯巴比妥灌胃造成肝硬化大鼠模型。该方法死亡率为24.1%，存活大鼠几乎全部为完全性肝硬化。我们对这只大鼠进行了70%的肝切除术，并测定了^3H-硫脒掺入量。正常组为37.3 ± <plus-minus>3.4cpm/gDNA，肝<plus-minus>硬化组为15.6 ± 3.4cpm/gDNA。因此，该模型的杠杆再生明显减少。接下来，我们检查了生长因子[转化生长因子α（TGF-α）、表皮生长因子（EGF）、胰岛素]对70%肝切除后肝再生的影响。皮下注射TGF-α对肝保护率70%的正常大鼠没有增加^3H-硫脒掺入。EGF也是如此。而肝硬化大鼠皮下注射TGF-α可显著增加^3H-缩氨酸掺入量。EGF和胰岛素联合注射也有同样的效果，TGF-α是由肝脏产生的，正常大鼠有足够的TGF-α，而肝硬化大鼠缺乏TGF-α，因此注射TGF-α可促进肝再生，这些生长因子的支持疗法可用于肝硬化肝切除术后促进肝再生。

The liver cirrhosis rat model was made by the oral intake of C CL_4 and phenobarbital. In this method, the mortality rate was 24.1%, and almost all the survived rats had compelete cirrhotic liver. We performed the 70% liver resection on this rats, and measured the ^3H-thimidine incorporation. This was 37.3<plus-minus>3.4cpm/gDNA in normal rat, whereas 15.6<plus-minus>3.4cpm/gDNA in liver cirrhosis rat model. So marked decrease of lever regeneration in that model was manifested. Next we examined the effect of growth factors [Transformining growth factor a (TGF-alpha), epidermal growth factor (EGF), insulin] to the liver regeneration after 70% liver resection. Subcutaneous injection of TGF-alpha on normal rat that was hepatected 70% did not increased the ^3H-thimidine incorporation. That of EGF was same result. But subcutaneous injection of TGF-alpha on the liver cirrhosis rat markedly increased the ^3H-thimidine incorporation. And the combined injection of EGF and insulin showed the same result.TGF-is made fromthe liver, so normal rat have enough amount of it, but liver cirrhosis rat lacks it. So the injection of TGF-alpha increased the liver regeneration.Support therapy of these growth factors may be used in the liver resection of cirrhosis liver to increase the liver regeneration.