Experimental Studies for Application of the Fixed Lung as an Oxygenator

固定肺作为氧合器应用的实验研究

• 批准号: 06671336
• 负责人: MORI Atsumi
• 金额: $ 1.54万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671336/
• 关键词: Fixed lung; Oxygenator; Gas exchange ability; Artificial lung; 灌流固定

The following studies were carried out for the purpose of developing an artificial lung from animal lung which has more durability and less antigenicity.1.Evaluation of gas exchange ability of fixed lung using a perfusion system with mechanical deoxygenator : An apparatus to preserve the resected lung by continuous perfusion with deoxygenated blood was developed. Free rabbit lungs which were fixed by the mixture of paraformaldehyde and glutaraldehyde (4% paraform-aldehyde+0.325% glutaraldehyde) were evaluated its gas exchange ability using this system. Two of 5 lungs were able to evaluate during 5 hour assessment period. D-PO2 (the difference of in-and out-flowing blood) were 28 and 40 torr at 5 hour of assessment (FiO2=O.21).2.Evaluation of gas exchange ability of fixed lung using an in vivo perfusion model : The left whole lung of canine was fixed by the same fixative in site. Gas exchange ability was evaluated under bilateral ventilation (Group I) and left single lungventilation (Group II). In Group I (n=3), all dogs were died at 2,3 and 4 hours of assessmsnt respectively. D-PO2 (the difference of arterial and venous blood) were 190,212 and 321 torr at the first assessment (FiO2=1.0). While, in Group II (n=3), 2 of 3 dogs were died just after ligation of right lung, and only one dog could be alive for 2 hour. D-PO2 at the first assessment was 158 torr.These findings suggest that the resected lung retains gas exchange ability comparable to that of the fresh lung if it has been fixed under appropriate conditions, but it is very difficult to flush out fixative from lung completely. Improvements in the method for fixation of the lung are desired, and, for this purpose, the completely. Improvements in the method for fixation of the lung are desired, and, for this purpose, the development of techniques that do not leave even a trace amount of the fixative or techniques for physical fixation is needed.

为研制更耐用、抗原性更低的动物肺人工肺，开展了以下研究工作：1.利用带机械除氧器的灌流系统评价固定肺的气体交换能力：研制了一种连续灌流脱氧血保存切除肺的装置。用多聚甲醛和戊二醛的混合物(4%多聚甲醛+0.325%戊二醛)固定兔肺，用该系统评价其气体交换能力。5个肺中有2个在5小时评估期内能够进行评估。D-PO2(流入和流出血液的差值)在评估5h时分别为28和40托(FiO2=0.21)。2.利用活体灌流模型评价固定肺的气体交换能力：用相同的固定剂原位固定犬的左全肺。分别在双侧通气组(I组)和左侧单肺通气组(II组)下评价气体交换能力。I组(n=3)分别于伤后2、3、4h处死。D-PO2(动脉血和静脉血的差值)分别为190、212和321托(FiO2=1.0)。而II组(n=3)，3只犬中有2只在右肺结扎后即刻死亡，仅1只能存活2小时。第一次评估的D-PO2为158托。这些结果表明，如果在适当的条件下固定，切除的肺仍能保持与新鲜肺相当的气体交换能力，但很难将固定剂完全从肺中排出。需要改进固定肺的方法，为此，需要完全。需要改进肺的固定方法，并且为此目的，需要开发甚至不留有微量固定剂的技术或用于物理固定的技术。

项目成果

S.Fujino: "Inhaled Nitric Oxide at the Time of Harvest Improves Early Lung Allograft Function." Ann Thorac Surg. (in press). (1997)

S.Fujino：“收获时吸入一氧化氮可改善早期同种异体肺移植功能。”

M.Yamashita: "Nicorandil,a potent adenosine triphosphate-sensitive potassium-channel openner,ameliorates lung allograft reperfusion injury." J Thorac Cardiovasc Surg. 112. 1307-1314 (1996)

M.Yamashita：“尼可地尔是一种有效的三磷酸腺苷敏感钾通道开放剂，可改善肺同种异体移植物再灌注损伤。”

S.Fujino: "Evaluation of Viability of Preserved Lung by Proton Magnetic Resonance Imaging" Transplantation Proceedings. 27. 783-785 (1995)

S.Fujino：“通过质子磁共振成像评估保留肺的活力”移植论文集。

S.Fujino: "Prharvest Nitroprusside Flush Improves Post-Transplant Lung Function." J Heart Lung Transplant. (in press). (1997)

S.Fujino：“Prharvest 硝普钠冲洗可改善移植后肺功能。”

M.Yamashita: "Nicorandil, a potent adenosine triphosphate-sensitive potassium-channel openner, ameliorates lung allograft reperfusion injury." J Thorac Cardiovasc Surg. 112. 1307-1314 (1996)

M.Yamashita：“尼可地尔是一种有效的三磷酸腺苷敏感钾通道开放剂，可改善肺同种异体移植物再灌注损伤。”