PTHmRNA and vitamin D_3 receptor RNA in primary hyperparathyroidism.

1.原发性甲状旁腺功能亢进症患者肾小管碳酸氢盐代谢本研究对10例甲状旁腺功能亢进症患者甲状旁腺切除术前后肾脏碳酸氢盐的酸碱平衡及肾脏处理情况进行了评价。但手术前后均未观察到酸碱平衡紊乱或改变。原发性甲状旁腺功能亢进症患者骨密度测定15例原发性甲状旁腺功能亢进症患者甲状旁腺切除术前后的骨密度变化。术后颅骨和腰椎骨密度即刻升高。而手术前后双侧肢体骨密度无明显变化。5例原发性甲状旁腺功能亢进症(甲状旁腺腺瘤)患者甲状旁腺总RNA的测定。然而，用Northern Bolt法测定甲状旁腺腺瘤的PTHmRNA和维生素D_3受体RNA均失败。我们得出结论：尽管甲状旁腺功能亢进症的肾小管碳酸氢盐阈值被抑制，而过多的内源性甲状旁腺激素增加了滤过负荷的部分尿碳酸氢盐排泄，但原发性甲状旁腺功能亢进症并未引起酸碱失衡。

1. Renal bicarbonate metabolism in patients with primary hyperparathyroidismThis study evaluated the acid-base balance and renal handling of bicarbonate in ten patients with primary hyperparathyroidism before and after parathyroidectomy.Renal tubular threshold of bicarbonate increased and fractional urinary bicarbonate excretion of the filtered load decreased after parathyroidectomy. However, no disturbances or changes in acid-base balance were observed before and after surgery.2. Bone mineral density in patients with primary hyperparathyroidismThis study evaluated the bone mineral density in 15 patients with primary hyperparathyroidism before and after parathyroidectomy.Bone mineral density decreased in the patient, with primary hyperparathyroidism of chemical and renal stone types. After surgery, bone mineral density of the skull and lumbar spine increased immediately. However, no changes in bone mineral density of the lower limbs were observed before and after surgery.3. PTHmRNA and vitamin D_3 receptor RNA in primary hyperparathyroidismTotal RNA of the parathyroid gland could be measured in five patients with primary hyperparathyroidism (parathyroid adenomas). However, the measurement of the PTHmRNA and vitamin D_3 receptor RNA in parathyroid adenomas using Northern bolt methods were failed.We conclude that primary hyperparathyroidism does not induce an acid-base disturbance, although the renal tubular threshold of bicarbonate is suppressed and fractional urinary bicarbonate excretion of filtered load is increased by excessiv endogenous PTH.Normal acid-base balance in primary hyperparathyroidism might be due to enhancement of extrarenal acid buffering capacyty (probably, bone).