Effects of the iontophoretically applied drugs using multi-barrel glass micro-pipette on the neural activities of the sacral micturition center in the rat.

在乌拉坦麻醉的Wistar大鼠上，观察了电离子导入神经递质对骶髓Onuf核神经活动的影响。电刺激阴部神经（持续时间30 μ s，10- 20 μ A，双脉冲：间隔5 ms，1- 20 Hz），记录逆行诱发电位。以潜伏期和对10 Hz双脉冲刺激的反应稳定为标准。将5支装有Pontamine天蓝（记录电极）、谷氨酸、去甲肾上腺素、5-羟色胺或生理盐水的Barret玻璃微吸管插入骶髓，观察它们对自发性兴奋和/或阴部神经对条件电刺激的神经反应的药理作用。5个测试的31个神经元兴奋的离子电渗应用谷氨酸和一个神经元抑制5-羟色胺。去甲肾上腺素对所测神经元有一定的作用。由于Onuf核记录技术的困难，本研究所获得的结果不是结论性的。然而，在这项研究中开发的方法应该是非常有用的功能研究的各种递质和药物的脊髓作用的排尿反流的神经机制。

Effects of iontophoretically injected neurotransmitters on the neural activities of Onuf's nucleus located in the sacral spinal cord were examined in uerthane anesthetized Wistar rats. The pudendal nerve was electrically stimulated (30mus in duration, 10-20muA,twin pulses : 5 ms interval at 1-20Hz) and the antidromically evoked potential was recorded. Stable latency and responses to the twin pulse stimulation at 10Hz was adopted the criteria for the antidromic response. Five barret glass glass micropipette filled with pontamine sky blue (recording electrode), glutamate, noradrenalin serotonin or saline was inserted into the sacral spinal cord and their pharmacological effects on the spontaneous discarges and/or neural responses to the conditioning electrical stimulation of the pudendal nerve were examined. Five of 31 neurons tested were excited by the iontophoretical application of glutamate and one neuron was inhibited by serotonin. Noradrenalin had to effects on the neuron tested. Because of the difficulties of recording technique from the Onuf's nucleus, the results obtained in the present study are not conclusive. However, the methodology developed in this study should be quite useful for the feature studies on the spinal action of the various transmitters and drugs on the neural mechanisms of micturition reflux.