Effects of the iontophoretically applied drugs using multi-barrel glass micro-pipette on the neural activities of the sacral micturition center in the rat.

多管玻璃微量移液器离子导入药物对大鼠骶骨排尿中枢神经活动的影响。

• 批准号: 06671607
• 负责人: NAKAGAWA Shuichi
• 金额: $ 0.26万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671607/
• 关键词: Onuf's nucleus; Sacral spiral cord; Rat; Glutamate; Noradrenalin; Serotonin; 脊髄; 球海面体筋

Effects of iontophoretically injected neurotransmitters on the neural activities of Onuf's nucleus located in the sacral spinal cord were examined in uerthane anesthetized Wistar rats. The pudendal nerve was electrically stimulated (30mus in duration, 10-20muA,twin pulses : 5 ms interval at 1-20Hz) and the antidromically evoked potential was recorded. Stable latency and responses to the twin pulse stimulation at 10Hz was adopted the criteria for the antidromic response. Five barret glass glass micropipette filled with pontamine sky blue (recording electrode), glutamate, noradrenalin serotonin or saline was inserted into the sacral spinal cord and their pharmacological effects on the spontaneous discarges and/or neural responses to the conditioning electrical stimulation of the pudendal nerve were examined. Five of 31 neurons tested were excited by the iontophoretical application of glutamate and one neuron was inhibited by serotonin. Noradrenalin had to effects on the neuron tested. Because of the difficulties of recording technique from the Onuf's nucleus, the results obtained in the present study are not conclusive. However, the methodology developed in this study should be quite useful for the feature studies on the spinal action of the various transmitters and drugs on the neural mechanisms of micturition reflux.

在超麻醉 Wistar 大鼠中检查了离子电渗注射神经递质对位于骶脊髓 Onuf 核神经活动的影响。电刺激阴部神经（持续时间30μs，10-20μA，双脉冲：1-20Hz间隔5ms）并记录逆向诱发电位。采用稳定的潜伏期和对 10Hz 双脉冲刺激的反应作为逆向反应的标准。将装有天蓝色（记录电极）、谷氨酸、去甲肾上腺素、血清素或盐水的五巴雷特玻璃微量移液器插入骶脊髓，检查它们对阴部神经条件性电刺激的自发放电和/或神经反应的药理作用。所测试的 31 个神经元中有 5 个因谷氨酸离子电渗疗法的应用而兴奋，而 1 个神经元则被血清素抑制。去甲肾上腺素对测试的神经元有影响。由于奥努夫核记录技术的困难，本研究获得的结果并不是结论性的。然而，本研究开发的方法对于各种递质和药物对排尿反流神经机制的脊髓作用的特征研究应该非常有用。