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The Factors Involved Invasive Ability of Endometrial Carcinoma Cells (Studies of Concerning Invasion, Metastasis of Endometrial Cancer)

子宫内膜癌细胞侵袭能力的影响因素（子宫内膜癌侵袭、转移的研究）

基本信息

批准号：
06671669
负责人：
KUDO Ryuichi
金额：
$ 0.26万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

A.The in vitro invasive ability, the expression of cell adhesion molecule E-cadherin, activity of matrix metalloproteinase (MMP) and K-ras point mutation were investigated in several human endometrial carcinoma cell lines.1) In vitro invasive abilities of endometrial carcinoma cell lines depend on the degree of cell differentiation and the origin of cell lines. A poorly-differentiated carcinoma cell line and a cell line derived from metastatic lymph node, or ascitis were more invasive than moderately and well-differentiated cell lines.2) Immunohistochemically, less invasive cell lines expressed E-cadherin strongly, where as a highly invasive cell line expressed E-cadherin weakly.3MMP-2 was secreted in serum-free conditioned medium of all cell lines. In an invasive cell line, the activity of MMP-2 was stronger than in other cell lines. And the activity of MMP-9 was detected in most invasive cell line.4) K-ras point mutation was not correlated with in vitro invasiveness of the tumor cells.B.The changes of the in vitro invasive ability after administration of anti E-cadherin antibody (HECD-1) were investigated in above cell lines.1) Immunohistochemically, expression of E-cadherin of less invasive cell lines changed to weak expression after administration of HECD-1.2) The invasive ability of less invasive cell lines increased after administration of HECD-1. However, that ability of highly invasive cell line was not changed even though these treatment.3) The ultrastructural features of less invasive cell lines changed to the similar findings of a highly invasive cell line after administration of HECD-1. These findings involved the decreasing cellular connection, pseudopolar cellular projection in invasing through matrigel and numerous microvilli.

A.研究了几种人子宫内膜癌细胞系的体外侵袭能力、细胞粘附分子E-cadherin的表达、基质金属蛋白酶（MMP）活性和K-ras点突变。1）子宫内膜癌细胞系的体外侵袭能力取决于细胞的分化程度和来源。低分化癌细胞系和转移性淋巴结或腹水来源的细胞系的侵袭性强于中分化和高分化细胞系; 2）免疫组化显示，低侵袭细胞系表达E-cadherin，而高侵袭细胞系表达E-cadherin较弱; 3所有细胞系的无血清条件培养液均分泌MMP-2。在侵袭性细胞系中，MMP-2的活性强于其他细胞系。K-ras基因点突变与肿瘤细胞体外侵袭能力无关。B.观察抗E-钙粘蛋白抗体（HECD-1）对上述细胞体外侵袭能力的影响。给予HECD-1后，侵袭性较低的细胞系的E-钙粘蛋白表达变为弱表达。2）给予HECD-1后，侵袭性较低的细胞系的侵袭能力增强。然而，即使这些处理也没有改变高侵袭性细胞系的能力。3）在施用HECD-1后，低侵袭性细胞系的超微结构特征改变为与高侵袭性细胞系相似的结果。这些发现包括细胞连接减少，假极性细胞突起侵入基质胶和大量的微绒毛。