The Factors Involved Invasive Ability of Endometrial Carcinoma Cells (Studies of Concerning Invasion, Metastasis of Endometrial Cancer)

a .研究了几种人子宫内膜癌细胞系的体外侵袭能力、细胞粘附分子E-cadherin的表达、基质金属蛋白酶（MMP）的活性和K-ras点突变。1)子宫内膜癌细胞系的体外侵袭能力取决于细胞分化程度和细胞系来源。低分化的癌细胞系和来自转移性淋巴结或腹水炎的细胞系比中等分化和高分化的细胞系更具侵袭性。2)免疫组化结果显示，侵袭性较弱的细胞系E-cadherin表达较强，侵袭性较强的细胞系E-cadherin表达较弱。所有细胞系无血清条件培养基中均有3MMP-2分泌。在侵袭细胞系中，MMP-2的活性强于其他细胞系。在大多数侵袭性细胞系中均检测到MMP-9的活性。4) K-ras点突变与肿瘤细胞的体外侵袭性无相关性。b .观察抗E-cadherin抗体（hed -1）对上述细胞系体外侵袭能力的影响。1)免疫组化结果显示，hed -1处理后，低创细胞株E-cadherin表达变为弱表达。1.2)hed -1处理后，低创细胞株的侵袭能力增强。然而，即使经过这些治疗，高侵袭性细胞系的这种能力也没有改变。3) hed -1处理后，低侵袭性细胞系的超微结构特征改变为高侵袭性细胞系的超微结构特征。这些发现包括细胞连接减少，通过基质和大量微绒毛侵入的假极细胞投射。

A.The in vitro invasive ability, the expression of cell adhesion molecule E-cadherin, activity of matrix metalloproteinase (MMP) and K-ras point mutation were investigated in several human endometrial carcinoma cell lines.1) In vitro invasive abilities of endometrial carcinoma cell lines depend on the degree of cell differentiation and the origin of cell lines. A poorly-differentiated carcinoma cell line and a cell line derived from metastatic lymph node, or ascitis were more invasive than moderately and well-differentiated cell lines.2) Immunohistochemically, less invasive cell lines expressed E-cadherin strongly, where as a highly invasive cell line expressed E-cadherin weakly.3MMP-2 was secreted in serum-free conditioned medium of all cell lines. In an invasive cell line, the activity of MMP-2 was stronger than in other cell lines. And the activity of MMP-9 was detected in most invasive cell line.4) K-ras point mutation was not correlated with in vitro invasiveness of the tumor cells.B.The changes of the in vitro invasive ability after administration of anti E-cadherin antibody (HECD-1) were investigated in above cell lines.1) Immunohistochemically, expression of E-cadherin of less invasive cell lines changed to weak expression after administration of HECD-1.2) The invasive ability of less invasive cell lines increased after administration of HECD-1. However, that ability of highly invasive cell line was not changed even though these treatment.3) The ultrastructural features of less invasive cell lines changed to the similar findings of a highly invasive cell line after administration of HECD-1. These findings involved the decreasing cellular connection, pseudopolar cellular projection in invasing through matrigel and numerous microvilli.