Mechanism of expression and biological function of sulfated glycolipids in the endometrium

本研究的目的是(1)分析子宫内膜中磺基转移酶(ST)和芳基硫酸酯酶(ASA)的定量变化，(2)定位硫化糖脂，(3)阐明硫化糖脂在子宫内膜细胞黏附中的作用。1.在正常子宫内膜中，ST活性在分泌期高于增殖期，而ASA活性在增生期高于分泌期。这些结果表明，子宫内膜中硫酸糖脂表达的变化与ST和AsA活性的变化有关。用35S示踪剂评估了来自子宫内膜癌、子宫颈癌和卵巢癌的肿瘤菌株中硫酸糖脂的表达。硫化糖脂在来自子宫内膜癌的肿瘤菌株中特异表达，其组成在不同菌株之间有所不同。硫脂…来自高分化癌症的Ishikawa菌株主要表达More，而来自中分化和低分化癌症的SNG-M和HEC108菌株分别表达乳糖脂，这表明分化程度与硫酸糖脂的表达有关。在来自子宫内膜癌的肿瘤菌株中，特异性地检测到ST活性。来自子宫内膜癌的菌株的AsA活性也往往更高，但与其他癌症相比并未显示出明显的增加。提示ST活性增强是银化糖脂表达的原因，也是子宫内膜癌的生物学特征。2.用一种新的针对硫化糖脂的单抗--硫脂，对子宫内膜中的硫化糖脂进行了免疫组织化学定位。糖脂主要存在于腺上皮细胞中。3.为探讨子宫内膜硫酸糖脂表达与性激素的关系，研究了雌激素和孕酮对石川株子宫内膜表达的影响。在雌激素和黄体酮存在的情况下，ST的活性增加了大约两倍。这一发现表明，性激素通过ST活性的改变参与了子宫内膜癌硫化糖脂表达的调节。较少

The objectives of the present study were to (1) analyze quantitative chages of sulfotransferase (ST) and arylsulfatase (ASA) in the endometrium, and (2) to localize sulfated glycollpids, and (3) to elucidate the contribution of sulfated glycolipids to cell adhesion in the endometrium. 1. When measured in the normal endometrium, ST activity was increased in the secretory phase compared to the proliferative phase, whilst ASA activity was increased in the proliferative phase compared to the secretory phase. These findings indicated that the changes of sulfated glycolipid expression in the endometrium involved alterations of ST and ASA activity. Expression of sulfated glycolipids in tumor strains derived from cancer of the endometrium, cancer of the uterine cervix, and ovarian cancer was assessed using a 35S-tracer. Sulfated glycolipids were specifically expressed in tumor strains derived from cancer of the endometrium and their composition varied between the different straings. Sulfatide … More was mainly expressed by the Ishikawa strain derived from well-differentiated cancer, whilst lactosylfatide was expressed by the SNG-M and HEC108 strains derived from moderately and poorly differentiated cancer, respectively, suggesting correlation between the degree of differentiation and the expression of sulfated glycolipids. ST activity was specifically detected in tumor strains derived from cancer of the endometrium. ASA activity also tended be higher in strains derived from cancer of the endometrium, but showed no significant increase compared to other cancers. These dindings suggested that enhanced ST activity was responsible for the expression of silfated glycolipids and was a biological characteristic of cancer of the endometrium. 2. A new monoclonal antibody directed againist a sulfated glycolipid, sulfatide, was used to immunohistochemically localize sulfated glycolipids in the endometrium. Glycolipids were found to be present mainly in the glandular epithelial cells. 3. To evaluate the relationship between endometrial expression of sulfated glycolipids and sex hormones, the effect of estrogen and progesterone on the Ishikawa strain was studied. ST activity was increased about two-fold in the presence of estrogen plus progesterone. This finding suggested that sex hormones were involved in mediating the expressin of sulfated glycolipids by cancer of the endometrium through changes in ST activity. Less