IMMUNOLOGICAL PATHOGENICITY OF GLYCOPROTEIN C-NEGATIVE HERPES SIMPLEX VIRUS TYPE I

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1.We isolated an extremely rare strains (TN1, TN2 and TN3) of herpes simplex virus type 1 (HSV-1) which were proven to be Glycoprotein C (gC) -negative by using anti-HSV-1 gC specific monoclonal antibodies. These viruses had a mutation in the gC gene resulting in a premature termination of gC translation, which was the cause of the gC-negative phenotype of the TN strains.2.We used the polymerase chain reaction to detect the virus genome in ocular samples from a patient with clinically diagnosed acute retinal necrosis. Primers specific for varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) were used. The VZV genome was detected in the aqueous humor but not in the vitreous from the patient. For HSV-1 primers, both the aqueous humor and the vitreous were negative. After detection of the virus genome, we used the PstI restriction endonuclease for VZV strain differentiation because the PstI-site-less variant was found in 25% of the strains of VZV in Japan. The VZV genome f … More rom the patient had the PstI cleavage site. These findings indicated that acute retinal recrosis studied here was thought to be caused by infection of the prevalent straing of VZV.3.Since Japanese patients diagnosed uveitis caused by Behcet's disease were described to have high levels of serum IgD,an investigation was taken to describe more fully the increased IgD levels in Japanese patients diagnosed uveitis. The study groups were composed of 110 subjects, which included 49 patients with Bahcet's disease, 15 patients with sarcoidosis, 10 patients with Vogt-Koyanagi-Harada disease, and 36 patients with the other uveitis. IgD measurements were performed on fresh serum samples or sera stored, using modifications of latex photometric immunoassay. Surface IgD (sIgD) expressions were determined and the correlation between the serum IgD levels and the percentage of sIgD was detected. Twelve of 110 study subjects had an elevated serum IgD.Of the 12,8 had Behcet's disease as was expected, and 4 had unclassified uveitis with retinal vasculitis who had been invited to the other uveitis group. These 4 patients had no other symptoms seen in Behcet's disease or hyperimmunoglobulinemia D (hyper IgD) syndrome. They were all young female with HLA-A-A24 (9) from the age of 8 to 25. A linear correlation was detected among them between the serum IgD levels and the percentage of sIgD.We considered the relationship between a hyper IgD state and retinal vasculitis of unclassified uveitis as well as Behcet's disease. Less