IMMUNOLOGICAL PATHOGENICITY OF GLYCOPROTEIN C-NEGATIVE HERPES SIMPLEX VIRUS TYPE I

糖蛋白C阴性单纯疱疹病毒I型的免疫致病性

• 批准号: 06671768
• 负责人: KUMANO Yuji
• 金额: $ 0.64万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671768/
• 关键词: Herpes simplex virus; Varicella-zoster virus; Acute retinal necrosis; Pst I; IgD; Behcet's disease; glycoprotein C; herpetic keratitis; acute retinal necrosis; varicella zoster; Pst I

1.We isolated an extremely rare strains (TN1, TN2 and TN3) of herpes simplex virus type 1 (HSV-1) which were proven to be Glycoprotein C (gC) -negative by using anti-HSV-1 gC specific monoclonal antibodies. These viruses had a mutation in the gC gene resulting in a premature termination of gC translation, which was the cause of the gC-negative phenotype of the TN strains.2.We used the polymerase chain reaction to detect the virus genome in ocular samples from a patient with clinically diagnosed acute retinal necrosis. Primers specific for varicella-zoster virus (VZV) and herpes simplex virus type 1 (HSV-1) were used. The VZV genome was detected in the aqueous humor but not in the vitreous from the patient. For HSV-1 primers, both the aqueous humor and the vitreous were negative. After detection of the virus genome, we used the PstI restriction endonuclease for VZV strain differentiation because the PstI-site-less variant was found in 25% of the strains of VZV in Japan. The VZV genome f … More rom the patient had the PstI cleavage site. These findings indicated that acute retinal recrosis studied here was thought to be caused by infection of the prevalent straing of VZV.3.Since Japanese patients diagnosed uveitis caused by Behcet's disease were described to have high levels of serum IgD,an investigation was taken to describe more fully the increased IgD levels in Japanese patients diagnosed uveitis. The study groups were composed of 110 subjects, which included 49 patients with Bahcet's disease, 15 patients with sarcoidosis, 10 patients with Vogt-Koyanagi-Harada disease, and 36 patients with the other uveitis. IgD measurements were performed on fresh serum samples or sera stored, using modifications of latex photometric immunoassay. Surface IgD (sIgD) expressions were determined and the correlation between the serum IgD levels and the percentage of sIgD was detected. Twelve of 110 study subjects had an elevated serum IgD.Of the 12,8 had Behcet's disease as was expected, and 4 had unclassified uveitis with retinal vasculitis who had been invited to the other uveitis group. These 4 patients had no other symptoms seen in Behcet's disease or hyperimmunoglobulinemia D (hyper IgD) syndrome. They were all young female with HLA-A-A24 (9) from the age of 8 to 25. A linear correlation was detected among them between the serum IgD levels and the percentage of sIgD.We considered the relationship between a hyper IgD state and retinal vasculitis of unclassified uveitis as well as Behcet's disease. Less

1.我们分离到一株极为罕见的单纯疱疹病毒Ⅰ型（HSV-1），经抗HSV-1糖蛋白C（gC）特异性单克隆抗体检测，证实为gC阴性。这些病毒在gC基因上发生突变，导致gC翻译提前终止，这是导致TN株gC阴性表型的原因。2.我们用聚合酶链反应检测了临床诊断为急性视网膜坏死患者的眼标本中的病毒基因组。使用水痘-带状疱疹病毒（VZV）和单纯疱疹病毒1型（HSV-1）特异性引物。VZV基因组在患者的房水中检测到，但在玻璃体中未检测到。对于HSV-1引物，房水和玻璃体均为阴性。在检测病毒基因组后，我们使用Pst I限制性内切酶对VZV毒株进行区分，因为在日本25%的VZV毒株中发现了Pst I位点缺失变异体。VZV基因组f ...更多信息 有Pst I切割位点。这些结果表明，急性视网膜再狭窄被认为是由流行株VZV感染引起的。3.由于被诊断为白塞病引起的葡萄膜炎的日本患者被描述为具有高水平的血清IgD，因此进行了一项调查，以更全面地描述被诊断为葡萄膜炎的日本患者中IgD水平的升高。研究组由110名受试者组成，其中包括49名Bahcet病患者，15名结节病患者，10名Vogt-Koyanagi-Harada病患者和36名其他葡萄膜炎患者。IgD测量新鲜血清样品或血清储存，使用乳胶光度免疫测定的修改。检测血清IgD水平与血清sIgD百分比的相关性。110例受试者中有12例血清IgD升高，其中8例为白塞病，4例为未分类的葡萄膜炎伴视网膜血管炎，他们被邀请到另一个葡萄膜炎组。这4例患者没有在白塞病或高免疫球蛋白血症D（高IgD）综合征中观察到的其他症状。均为HLA-A-A24（9）年轻女性，年龄8 ~ 25岁。血清IgD水平与sIgD百分比呈直线相关，我们认为高IgD状态与未分类葡萄膜炎视网膜血管炎以及白塞病之间存在关系。少

项目成果

Yuji Kumano, Junichi Manabe, Mika Hamamoto, Yoh-Ichi Kawano, shigeru Sugai, Toshiaki Kubota, and Hajime Inomata.: "Polymerase chain reaction and PstI restriction endonuclease pattern for the detection of varicella-zoster virus genome in ocular samples fro

Yuji Kumano、Junichi Manabe、Mika Hamamoto、Yoh-Ichi Kawano、shigeru Sugai、Toshiaki Kubota 和 Hajime Inomata.：“用于检测眼部样本中水痘带状疱疹病毒基因组的聚合酶链式反应和 PstI 限制性内切核酸酶模式

熊野祐司: "桐沢型ぶどう膜炎患者の前房水から検出された水痘帯状ヘルパスウイルスゲノムのPstI制限酵素による解析" 臨床眼科. 49. 427-430 (1995)

Yuji Kumano：“使用 PstI 限制性内切酶分析从 Kirisawa 型葡萄膜炎患者的前房水中检测到的水痘带状疱疹病毒基因组”《临床眼科》49. 427-430 (1995)。

Yuji Kumano, Junichi Manabe, Mika Hamamoto, Yoh-Ichi Kawano, Hiroko Minagawa, Yasuyuki Fukumaki, and Hajime Inomata.: "Detection of varicella-zoster virus genome having a Pst I site in the ocular sample from a patient with acute retinal necrosis." Ophthal

Yuji Kumano、Junichi Manabe、Mika Hamamoto、Yoh-Ichi Kawano、Hiroko Minakawa、Yasuyuki Fukumaki 和 Hajime Inomata：“在急性视网膜坏死患者的眼部样本中检测到具有 Pst I 位点的水痘带状疱疹病毒基因组。

Yuji Kumano: "Detection of varicella-zoster virus genome having a Pstl site in the ocular sample from a patient with" Ophthalmic Research. 27. 310-316 (1995)

Yuji Kumano：“在眼科研究患者的眼部样本中检测到具有 Pstl 位点的水痘带状疱疹病毒基因组”。

熊野祐司: "桐沢型ぶとう膜炎患者の前房水から検出された水痘帯状ヘルペスウイルスゲノムのPstl制限酵素による解析" 臨床眼科. 49. 427-430 (1995)

Yuji Kumano：“使用 Pstl 限制酶分析从 Kirisawa 型葡萄膜炎患者的前房水中检测到的水痘带状疱疹病毒基因组”《临床眼科》49. 427-430 (1995)。