Prenatal Platelet Genotyping for Neonatal Alloimmune Thrombocytopenia.

新生儿同种免疫血小板减少症的产前血小板基因分型。

• 批准号: 06672298
• 负责人: OHTA Hitoshi
• 金额: $ 1.09万
• 依托单位: Fukushima Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672298/
• 关键词: Neonatal alloimmune thrombocytopenia; Anti-platelet antibody; PCR-SSP; Amniotic fluid cells; HPA-4; Genotype; Prenatal diagnosis; fetus; Platelet; Alloimmunization; DNA診断; 遺伝子増幅; 血小板型; プライマー

To reveal the prevalence of maternal alloimmunization to platelet antigens (HPA) and the incidence of neonatal alloimmune thrombocytopenia (NAT), 10,000 maternal sera were tested for the presence of anti-HPA antibodies using the Mixed Passive Haemagglutination. Ninety-six anti-HPA were found ; anti-HPA-4b, -5a, -5b and anti-Nak^a in 23,2,70 and one pregnant women, respectively.Optimal management of NAT requires the determination of fetal HPA status as early as possible. We explored the feasibility of fetal HPA-4 genotyping by amplification of DNA from amniotic cells using sequence specific primers-polymerase chain reaction (PCR-SSP). Three babies in which fetal HPA-4 type from amniotic fluid cells was completely concordant with type using the blood of these infants studied. Two fetuses were shown to be homozygous PHA-4a/a, and had normal platelet counts. The third fetus was heterozygous HPA-4a/b incompatible with maternal antibody, and developed thrombocytopenia. These findings suggest that HPA-4 genotyping can be reliable determinied from amniotic fluid by DNA amplification.

为了揭示母体对血小板抗原（HPA）的同种免疫的流行率和新生儿同种免疫性血小板减少症（NAT）的发生率，使用混合被动血凝试验检测了10，000份母体血清中抗HPA抗体的存在。抗HPA抗体阳性96例，抗HPA-4 b抗体阳性23例，抗HPA-5a抗体阳性2例，抗HPA-5 b抗体阳性70例，抗Nak ^a抗体阳性1例。我们探讨了应用序列特异性引物-聚合酶链反应（PCR-SSP）扩增羊膜细胞DNA进行胎儿HPA-4基因分型的可行性。3例羊水细胞中胎儿HPA-4型与自身血中HPA-4型完全一致。两个胎儿显示为纯合子PHA-4a/a，血小板计数正常。第三胎为杂合子HPA-4a/B，与母体抗体不相容，并出现血小板减少症。结果表明，DNA扩增法可用于羊水中HPA-4基因分型。

项目成果

H. Ohto, K. Kato, Y. Tohyama, R. Motoki, A. Sato, et al: "Prenatal Diagnosis of Human Platelet Antigen Type4." Transfusion Science. 17. (1996)

H. Ohto、K. Kato、Y. Tohyama、R. Motoki、A. Sato 等人：“人类血小板抗原 4 型的产前诊断”。

加藤久美子,大戸斉: "羊水細胞を用いた新生児血小板型の出生前診断" 日本輸血学会雑誌. 42. (1996)

加藤久美子 (Kumiko Kato)、小藤仁 (Hitoshi Oto)：“利用羊水细胞对新生儿血小板类型进行产前诊断”，日本输血学会杂志 42。(1996)

加藤久美子、遠山ゆり子、大戸斉、元木良一他: "胎児血小板遺伝子型の羊水を用いた出生前診断" 日本輸血学会雑誌. 42. (1996)

Kumiko Kato、Yuriko Toyama、Hitoshi Oto、Ryoichi Motoki 等：“使用羊水进行胎儿血小板基因型的产前诊断”日本输血学会杂志 42。（1996 年）

H. Ohto, K. Kato, Y. Tohyama, R. Motoki, A. Sato, et al: "Prenatal Diagnosis of Human Platelet Antigen Type4." Transfusion Science. 17. (1996)加藤久美子,遠山ゆり子、大戸斉、元木良一他:

H. Ohto、K. Kato、Y. Tohyama、R. Motoki、A. Sato 等人：“人类血小板抗原 4 型的产前诊断”。输血科学 17。(1996) Kumiko Kato、Yuriko Toyama、Hitoshi Ohto，本木良一等人：

大戸斉 他: "Prenatal Determination of Human Pletelet Antigen Type 4." Transfusion Science. 17. (1996)

Hitoshi Ohto 等人：“人类血小板抗原 4 型的产前测定。”17。（1996）