Studies on Changes of Urinary Protein Components and Alkaline Phosphatase as Spotrs-Related Stress Markers in Growth Phase

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In order to gain a fuller understanding of soprts-related stress markers, we studied urinary protein components and alkaline phosphatase (ALP) levels and isozyme patterns before and after strenous exercise. The urinary protein concentrations and ALP activity-peaks were seen 5-15 minutes and 1 hour after running. SDS-PAGE and Western blotting showed that albumin was the main protein band in the urine which was collected before and 3 houes after running. However, alpha2-macrogloblin, immunogloblin G and transferrin wer seen in the peak frction of total protein concentration, in addition to albumin. While levels of ALP of higher molecular mass were observed to increase slightly, following intense exerclse, neither the intensity nor the duration of the exercise appeared to have an overly significant effect on them.After buthanol extraction, a distinct band of ALP activity was observed in the polyacrylamide gel, having the same degree of mobility as those of the renal cortex and medulla.A portion of post-exercise urinary ALP was considered to have originated from the kidney. As ALP is now understood to be one of the markers of bone metabolism, urinary ALP will most certainly come under ever more intense scrutiny, as regards its connection to sports-related stress and osteoporosis.