Studies on Changes of Urinary Protein Components and Alkaline Phosphatase as Spotrs-Related Stress Markers in Growth Phase

生长期尿蛋白成分和碱性磷酸酶作为斑点相关应激标志物变化的研究

• 批准号: 06680091
• 负责人: SUGIMOTO Hideo
• 金额: $ 1.28万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680091/
• 关键词: sports-related stress markers; urinary protein components; alkaline phosphatase; 尿成分; アルカリフォスファターゼ; 高分子アルカリフォスファターゼ; PAGE; 運動; 過剰運動; 成長期

In order to gain a fuller understanding of soprts-related stress markers, we studied urinary protein components and alkaline phosphatase (ALP) levels and isozyme patterns before and after strenous exercise. The urinary protein concentrations and ALP activity-peaks were seen 5-15 minutes and 1 hour after running. SDS-PAGE and Western blotting showed that albumin was the main protein band in the urine which was collected before and 3 houes after running. However, alpha2-macrogloblin, immunogloblin G and transferrin wer seen in the peak frction of total protein concentration, in addition to albumin. While levels of ALP of higher molecular mass were observed to increase slightly, following intense exerclse, neither the intensity nor the duration of the exercise appeared to have an overly significant effect on them.After buthanol extraction, a distinct band of ALP activity was observed in the polyacrylamide gel, having the same degree of mobility as those of the renal cortex and medulla.A portion of post-exercise urinary ALP was considered to have originated from the kidney. As ALP is now understood to be one of the markers of bone metabolism, urinary ALP will most certainly come under ever more intense scrutiny, as regards its connection to sports-related stress and osteoporosis.

为了更全面地了解运动相关的应激标志物，我们研究了大强度运动前后尿蛋白组分和碱性磷酸酶（ALP）水平及其同工酶谱。尿蛋白浓度和ALP活性峰值分别出现在跑后5-15 min和1h。SDS-PAGE和Western blotting结果显示，在跑前和跑后3 h收集的尿液中，白蛋白是主要的蛋白条带。除白蛋白外，α 2-巨球蛋白、免疫球蛋白G和转铁蛋白也出现在总蛋白浓度的峰值频率。高分子量的ALP在剧烈运动后略有增加，但运动强度和运动时间对ALP的影响均不显著，丁醇提取后，在聚丙烯酰胺凝胶中观察到一条明显的ALP活性带，运动后尿碱性磷酸酶的一部分被认为来源于肾脏。由于ALP现在被认为是骨代谢的标志物之一，尿ALP肯定会受到更严格的审查，因为它与运动相关的压力和骨质疏松症有关。

项目成果

Toyoji Sato,Hideo Sugimoto Masaharu Yamamoto et al: "Studies on the urinary macromolecular alkaline phosphatases" がん新病誌. 34(1). 35-37 (1995)

Toyoji Sato、Hideo Sugimoto Masaharu Yamamoto 等人：“尿大分子碱性磷酸酶的研究”，癌症新疾病杂志 34(1)。

Hideo Sugimoto, Toyoji Sato, Masaharu Yamamoto, et al: "The significance of urinary alkaline phosphatase in relation with the exercise" MEMOIRS of THE FACULTY OF Niigata University. 36-2. 159-163 (1995)

Hideo Sugimoto、Toyoji Sato、Masaharu Yamamoto 等人：“尿碱性磷酸酶与运动的重要性”新泻大学学院回忆录。

Toyoji Sato, Hideo Sugimoto, Masaharu Yamamoto, et al: "Studies on the urinary macromolecular alkaline phosphatases" Journal of Niigata Cancer Center Hospital. 34-1. 55-57 (1995)

Toyoji Sato、Hideo Sugimoto、Masaharu Yamamoto 等：“尿大分子碱性磷酸酶的研究”新泻癌症中心医院杂志。

Toyoji Sato,Hideo Sugimoto et al: "L-cysteine sensitive,tissue-unspecific variant alkaline phosphatases detected in the case of lung cancer" がん新病誌. 33(2). 35-37 (1994)

Toyoji Sato、Hideo Sugimoto 等人：“在肺癌病例中检测到的 L-半胱氨酸敏感性、组织非特异性变异碱性磷酸酶”《癌症新疾病杂志》33(2) (1994)。

Toyoji Sato.Hideo Sugimoto Masaharu Yamamoto,et al: "Alkaline phosphatase isozymes of aerand and urine and arinary protein in young man bepore and apten running 3km" Eur J Appl Physiol.69. 355-360 (1994)

Toyoji Sato.Hideo Sugimoto Masaharu Yamamoto 等人：“跑 3 公里的年轻人中空气和尿液的碱性磷酸酶同工酶以及空气蛋白”Eur J Appl Physiol.69。