Simultaneous removal of nitrogen and non biodegradable substances in the treatment of leachate from sea-based solid waste disposal site

对驯化海洋硝化污泥（AMNS）对挥发性脂肪族氯代烃的降解能力进行了实验研究。AMNS对氯仿（CF）、三氯乙烯（TCE）、1,1,2-三氯乙烷（1,1,2- tca）和1,1,2,2-四氯乙烷具有降解能力，但对C-H键被氯原子高度取代的1,1,1-三氯乙烷和四氯乙烷（PCE）没有降解能力。氨是硝化菌降解CF、TCE、1,1,2- tca和PCA所需的主要底物。四氯化碳（CT）在好氧条件下也能被AMNS降解，但被AMNS絮凝体厌氧部分的厌氧细菌降解。广泛分布于各种细菌絮凝体中的厌氧菌可以很容易地完成CT的生物降解。根据挥发性脂肪族氯代烃的生物降解性和对氨氮硝化的抑制作用，提出了微生物降解挥发性脂肪族氯代烃的四种类型。采用聚乙烯醇（PVA）制备的固定化AMNS微球，对某海基固体废物处理场的渗滤液进行了连续硝化处理实验，并考察了磷酸三酯的去除情况。三（2-氯乙基）磷酸（TCEP）和三（3-氯丙基）磷酸（TCPP）具有较高的水溶性，在AMNS固定化微球对渗滤液的连续硝化实验中得到了较好的去除效果。这些磷酸三酯被AMNS生物降解证明发生在这些磷酸三酯在PVA颗粒上的部分吸附。

Biodegradation capabilities of acclimated marine nitrifying sludge (AMNS) for volatile aliphatic chlorinated hydrocarbons were experimentally investigated. AMNS had the capability to degrade chloroform (CF), trichloroethylene (TCE), 1,1,2-trichloroethane (1,1,2-TCA) and 1,1,2,2-tetrachloroethane but had not 1,1,1-trichloroethane and tetrachloroethylene (PCE) whose C-H bonds were highly substituted by chlorine atoms. Ammonia, which is the main substrate for nitrifiers, was required for the degradation of CF,TCE,1,1,2-TCA and PCA by AMNS.Carbon tetrachloride (CT) was degraded by the AMNS even under aerobic condition, but was demonstrated to be degraded by the anaerobic bacteria which exist in the anaerobic portion of AMNS floc. CT biodegradation was readily accomplished by the anaerbic bacteria which are widely distributed in various kinds of bacterial flocs. Four types of classification for the microbial degradation of volatile aliphatic chlorinated hydrocarbon were proposed based on their biodegradabilities and inhibitory effects on nitrification of AMNS.By using immobilized AMNS pellets which were prepared by polyvinylalcohol (PVA), continuous nitrification treatment experiments of leachate from a sea-based solid waste disposal site were carried out and the removal profiles of phosphoric acid triesters were investigated. Tris (2-chloroethyl) phosphate (TCEP) and tris (3-chloropropyl) phosphate (TCPP), which have high water solubility, were effectively removed during the continuous nitrification experiments of leachate by AMNS immobilized pellets. Biodegradation of these phosphoric acid triesters by AMNS was demonstrated to occur with partial adsorption of these phosphoric acid triesters on PVA pellets.