The role of cytokines in autoimmune encephalomyelitis and rejection processes of neural grafts

细胞因子在自身免疫性脑脊髓炎和神经移植排斥过程中的作用

• 批准号: 06680751
• 负责人: MATSUMOTO Yoh
• 金额: $ 0.45万
• 依托单位: Tokyo Metropolitan Institute for Neuroscience
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680751/
• 关键词: Autoimmune encephalomyelitis; Neural graft; Cytokines; Quantitative PCR; in situ RT-PCR; in situ RT-PCR

In the present study, we investigated the role of cytokines secreted in the lesion in the central nervous system (CNS) and cytokine network.Since the amount of cytokine protein and mRNA expressed in the CNS is very little, it was very difficult to examine them by ordinary methods such as Western and Northern blot analysis.Instead, using the experimental autoimmune encephalomyelitis (EAE) and neural tissue grafting model, we tried to establish the quantitative analysis of cytokine mRNA after amplification by the PCR techniques, i.e., competitive PCR.In acute and monophasic rat EAE,both pro-inflammatory )IFN-gamma and TNF-alpha) and anti-inflammatory (IL-10) cytokines reached a maximal at the early stage of EAE suggesting the nature of its clinical course.On the other hand, in the graft rejection processes, mRNA for cytolytic protein such as perforin and granzyme B was expressed markedly.These disease-specific cytokine secretion profile strongly suggest that the nature of the lesion formed in the CNS regulates the mode of the lesion repair by modulating the cytokine network formed in the brain.

本研究利用实验性自身免疫性脑脊髓炎（EAE）和神经组织移植模型，探讨了中枢神经系统（CNS）病变中分泌的细胞因子及其网络的作用。由于CNS中表达的细胞因子蛋白和mRNA量很少，用常规方法如Western和北方印迹分析很难检测到。我们试图建立通过PCR技术扩增后细胞因子mRNA的定量分析，即，在急性和非急性期大鼠EAE中，促炎性（IFN-γ和TNF-α）和抗炎性（IL-10）细胞因子在EAE的早期达到最大值，表明其临床过程的性质。另一方面，在移植排斥过程中，细胞溶解蛋白如穿孔素和颗粒酶B的mRNA表达显著。特异性细胞因子分泌谱强烈提示CNS中形成的损伤的性质通过调节脑中形成的细胞因子网络来调节损伤修复的模式。

项目成果

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