MAPPING OF bg GENE IN RAT USING MICROSATELLITE MARKER.

使用微卫星标记绘制大鼠 bg 基因图谱。

基本信息

  • 批准号:
    06680828
  • 负责人:
  • 金额:
    $ 0.96万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

Chediak-Higashi syndrome (CHS) is an autosomal recessive genetic disease that has been reported in human, mink, cats, and mice. CHS was initially recognized as a disorder in which melanocytes, neutrophils, and lymphocytes contained giant cytoplasmic granules. The mutation of mice comparable to human CHS is named "beige " from the diluted coat color. If CHS rats are available, they are probably useful for investigations such as biochemical analysisof CHS.We found that the coat color mutant DA strain maintained in Hamamatsu University School of Medicine was comparable to human CHS and it was named DA-bg. In this research we attempted to mapping bg gene on rat chromosome. The following results were obtained,The mouse Chr.13 is comparable to the rat Chr.17 so, we collected 8 of microsatellite marker (MSM). The 6 of MSM were amplified by PCR method. The 10 of inbred strains of rats were tested by these MSM and it was found polymorphism between DA-bg and BN at two (Prolactin : PRL and Cholinergic recepter, muscaric3 : ACRM) of MSM.In 80 progeny of {(DA-bg*BN)*DA-bg} backcross, it was found linkage between PRL to bg (30cM), ACRM to bg (23cM), and PRL to ACRM (11 cM). It was confirmed that the bg gene was on Chr.17 in rat.
Chediak-Higashi综合征(CHS)是一种常染色体隐性遗传病,在人类、水貂、猫和小鼠中均有报道。CHS最初被认为是一种黑色素细胞、中性粒细胞和淋巴细胞含有巨大细胞质颗粒的疾病。与人类CHS相当的小鼠突变被命名为“米色”,因为被稀释的毛色。如果CHS大鼠可用,它们可能对CHS的生化分析等调查有用。我们发现滨松大学医学院维持的毛色突变菌株DA与人类CHS相当,并将其命名为DA-bg。本研究试图在大鼠染色体上定位bg基因。得到的结果如下:小鼠的Chr.13与大鼠的Chr.17相当,因此我们收集了8个微卫星标记(MSM)。用PCR法扩增6株MSM。对10个自交系大鼠进行MSM检测,发现DA-bg和BN在MSM的两个位点(催乳素:PRL和胆碱能受体muscaric3: ACRM)存在多态性。在80个{(DA-bg*BN)*DA-bg}回交后代中,发现PRL与bg (30cM)、ACRM与bg (23cM)、PRL与ACRM (11cm)之间存在连锁。在大鼠中证实了bg基因位于Chr.17。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nishikawa, T Nishimura, M: "Genetic relationships of the 14 inbred strains of rats according to the composition of biochemical marker genes." Medicine and Biology. 132. (1996)
Nishikawa, T Nishimura, M:“根据生化标记基因的组成,了解 14 种近交系大鼠的遗传关系。”
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Nishikawa, T Nishimura, M: "A rapid and easy electrophoretic method for detecting of biochemical loci of rat." Exp.Anim.45. (1996)
Nishikawa, T Nishimura, M:“一种快速简便的电泳方法,用于检测大鼠的生化位点。”
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西川哲、西村正彦: "生化学的標識遺伝子組成から見た14近交系ラットの遺伝学的位置" 医学と生物学. 132. (1996)
Tetsu Nishikawa,Masahiko Nishimura:“从生化标记基因组成的角度看 14 只近交大鼠的遗传位置”医学与生物学 132。(1996)
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Nishikawa,T.: "A Rapid and Easy Electrophoretic Method for Detecting Biochemical loci of Rat(Rattus norvegicus)" Exp. Anim.45. (1996)
Nishikawa,T.:“一种快速简便的电泳方法检测大鼠(Rattusnorvegicus)的生化位点”实验。
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Nishikawa,T Nishimura,M: "A Rapid and Easy Electrophoretic Method for Detecting of Biochemical Loci of Rat." Exp.Anim.45. (1996)
Nishikawa,T Nishimura,M:“一种快速简便的电泳方法检测大鼠生化位点。”
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