A diagnostic study of lectin binding pattern and color variation in oral mucosal epithelium on diseases of the oral mucosa

许多口腔粘膜疾病病因不明。临床表现为大疱性、糜烂性、角化性、溃疡性病变，本研究的目的是观察扁平苔藓（OLP）上皮细胞膜与麦胚凝集素（WGA）的结合情况，以及不同临床类型OLP上皮细胞膜颜色的变化。正常口腔粘膜上皮细胞凝集素结合强度较强，而OLP各临床类型上皮细胞凝集素结合强度较弱，但不规则，尤其是糜烂型上皮细胞凝集素结合强度明显降低。2）用CHROMA仪CS-100（MINOLTA）测定上皮细胞的颜色变化。颜色空间（CIE 1976 L^<**>a^<**>B^<**>）和色差（CIE 1976 DELTAE^**>ab），与正常粘膜上皮相比，颜色空间L^**>和B^**>为负值，3）采用视觉模拟评分法（VAS）对OLP引起的疼痛进行定量测量。各临床类型评分均有不同程度的变化，且随临床治疗而改善。颜色变化与VAS评分呈相关性变化。

Many kinds of diseases of oral mucosa are unknown etiology. Usually clinical pattern shows bullous, erosive, keratinize, ulcerative leasions, so leading to differential diagnosis is difficult.The purpose of in this study was to examine possible epithelial cell membrane changes in lichen planus affected by the use of the WGA and the color variation in various clinical types of the lichen planus (OLP).The results were obtained as follows.1) The binding of the epithelium to wheat germ agglutinin (WGA) was examined.In healthy oral mucousal epithelium, the binding pattern was obserbed regularly and strong but in OLP irregularly and weak in various clinical types especialy most decrease in lectin binding was observed in erosive lesion.2) We measured the color variation of the epithelium by CHROMA meter CS-100(MINOLTA). The color space (CIE 1976 L^<**>a^<**>b^<**>) and the color difference (CIE 1976 DELTAE^<**>ab) were examined.Compare to healthy mucousal epithelium with lichen planus, the color space L^<**> and b^<**> were minus quantity, a^<**> was plus quantity.The color differrence DELTAE^<**>ab was higher about 7th levels.3) We measured the pain caused by OLP quantativery, using a visual analogue scale (VAS). The score showed a change in various clinical types and better by clinical therapies. The color variation and VAS showed corelatively changes.