Do trypanosomes control mRNA export?

锥虫控制 mRNA 输出吗？

• 批准号: 447334523
• 负责人: Professorin Dr. Susanne Kramer
• 金额: --
• 依托单位: Lehrstuhl für Zell- und Entwicklungsbiologie (Zoologie I)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/447334523?language=en
• 关键词: Do; trypanosomes; control; mRNA; export

In eukaryotes, mRNAs are transcribed in the nucleus, but serve as templates for protein production in the cytoplasm. Thus, every mRNA needs to pass the double-membraned barrier that surrounds the nucleus: the nuclear envelope. It is believed that eukaryotes control mRNA export, to prevent immature or faulty mRNAs from reaching the cytoplasm to prevent the production of potentially toxic proteins. Consistent with this view, elaborate mRNA export control systems were identified in yeast and human. Trypanosomes left the eukaryotic mainstream early and lack homologues to many of the components of these export control systems. Moreover, we recently found that trypanosomes can start nuclear export even though transcription and mRNA processing are not finished. Thus, trypanosomes either have a much simpler mRNA export control system, or perhaps none.We plan to study nuclear export control in trypanosomes in a systematic way. We will screen for changes in mRNA export that occur after experimental manipulation of RNA features or proteins with a suspected function in export control. We will use single molecule in situ hybridisation as the major method and confirm important results by qPCR and RNA sequencing of nuclear and cytoplasmic RNAs. Our experiments will answer the question, whether mRNA export control is a feature of all eukaryotes, or whether it has evolved only in a subgroup at a later stage in eukaryotic evolution. In case trypanosomes have a simpler mRNA export system, we will have identified its main features and this way contribute to a better understanding of the evolution of mRNA export control in eukaryotes.

在真核生物中，mrna在细胞核中转录，但在细胞质中作为蛋白质生产的模板。因此，每个mRNA都需要通过包围细胞核的双层膜屏障：核膜。据信真核生物控制mRNA的输出，以防止未成熟或有缺陷的mRNA到达细胞质，以防止产生潜在的有毒蛋白质。与这一观点一致的是，在酵母和人类中发现了复杂的mRNA出口控制系统。锥虫很早就离开了真核生物的主流，并且与这些出口管制系统的许多组成部分缺乏同源物。此外，我们最近发现，即使转录和mRNA加工尚未完成，锥虫也可以开始核输出。因此，锥虫要么有一个更简单的mRNA输出控制系统，要么可能没有。我们计划对锥虫的核出口管制进行系统的研究。我们将筛选在实验操作具有可疑出口控制功能的RNA特征或蛋白质后发生的mRNA出口变化。我们将以单分子原位杂交为主要方法，并通过qPCR和细胞核和细胞质RNA的RNA测序来确认重要结果。我们的实验将回答这个问题，mRNA出口控制是所有真核生物的特征，还是它只在真核生物进化的后期阶段的一个亚群中进化。如果锥虫有一个更简单的mRNA输出系统，我们将确定其主要特征，这有助于更好地理解真核生物mRNA输出控制的演变。