Extracellular RNAs in vascular wilt diseases

引起维管枯萎的病原体是最具破坏性的植物线材之一。主要由土壤传播的卵菌、细菌或真菌寄生在输水的木质部导管上，导致供水受损、枯萎，最终导致植物死亡。血管性枯萎病和寄主防御反应的分子基础尚不清楚，主要是因为病原菌存在于寄主植物内部，不易接近。在这个项目中，我们主要想把重点放在甘蓝型油菜-长孢黄萎病相互作用作为一个研究系统，因为它可以评估作为宿主-真菌战场的植物脉管系统。此外，还将比较苛养木杆菌和长孢弧菌感染对拟南芥胞外增殖反应的影响。该策略的目的是阐明在芸苔科与血管性枯萎病病原体相互作用中胞外RNA - RBP复合物交换的分子机制。研究主要围绕以下问题展开：(a)植物寄主与血管性枯萎病病原菌之间的胞外RNA交换如何参与侵染过程；(b)寄主与病原菌之间胞外RNA和RNA- rbp复合物如何被靶向并交换。我们的研究将揭示细胞外rna和RNA-RBP复合物是否在芸苔科植物与真菌和细菌血管性枯萎病病原体的相互作用中发挥作用。结合联盟中其他项目的见解，我们努力寻找增加植物抗性的新方法。

Pathogens inducing vascular wilts are among the most destructive plant threads. The mostly soil-borne oomycetes, bacteria, or fungi colonize the water-conducting xylem vessels what leads to impairment of water supply, wilting, and eventually plant death. The molecular basis underlying vascular wilt diseases and host defense reactions is not well understood, mainly because the pathogens reside in the interior of their host plants and are not easy to access. In this project we want to focus mainly on the Brassica napus - Verticillium longisporum interaction as a research system, because it allows assessing the plant vasculature as the host-fungus battleground. In addition, effects of Xylella fastidiosa and V. longisporum infection on the apoplastic response in Arabidopsis will be compared. The goal of this strategy is to elucidate the molecular mechanism of the exchange of extracellular RNA – RBP complexes in the interaction between Brassicaceae and vascular wilt pathogens. The research is centered around the questions (a) how extracellular RNA exchange between plant host and vascular wilt pathogens is involved in the infection process, (b) how extracellular RNAs and RNA-RBP complexes can be targeted and exchanged between hosts and pathogens. Our studies will reveal if extracellular RNAs and RNA-RBP complexes play a role in the interaction of Brassicaceae plants with a fungal and a bacterial vascular wilt pathogen. Combined with the insights from the other projects in the consortium, we strive for finding novel ways to increase plant resistance.