Roles of multifunctional adaptor proteins, GGAs, in membrane traffic

在本研究项目中，我们对GAs及其相关的Tom1家族蛋白进行了如下研究：(1)我们发现Arfs与GGAs的GAT结构域的N-GAT亚域结合。(2)通过X射线结晶学，我们揭示了GGA3-GAT结构域与ARF的复合体的三维结构。(3)我们发现泛素与GGaS和Tom1家族蛋白的GAT结构域的C-GAT亚域结合。(4)通过X射线结晶学，我们揭示了GGA3-GAT结构域和Tom1-GAT结构域在泛素复合物中的三维结构。(5)我们发现Tollip(Toll相互作用蛋白)与Tom1家族蛋白的GAT结构域相互作用。(6)我们发现Tollip将Tom1家族蛋白和泛素化蛋白招募到内体。

In this research project, we revealed the followings on GGAs and its related Tom1 family proteins.(1)We found that ARFs bind to the N-GAT subdomain of the GAT domain of GGAs.(2)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain in complex with ARF.(3)We found that ubiquitin bind to the C-GAT subdomain of the GAT domain of GGAs and Tom1 family proteins.(4)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain and that of the Tom1-GAT domain in complex with ubiquitin.(5)We found that Tollip (Toll-interacting protein) interacts with the GAT domain of the Tom1 family proteins.(6)We revealed that Tollip recruits Tom1 family proteins and ubiquitinated proteins onto endosomes.