Q-ToF-Massenspektrometer

Q-ToF质谱仪

• 批准号: 449879597
• 金额: --
• 依托单位: Ruprecht-Karls-Universität Heidelberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/449879597?language=en
• 关键词: ToF; Massenspektrometer

Proteomics encompasses the analysis of the entire protein complement of a biological sample including all posttranslational modifications. As such, it defines the complex phenotype of functional genomics. Proteome analytics is most challenging in modern life sciences, because of the large dynamic range and variability of proteins. Advanced proteome analytics requires the application of highly complex mass spectrometry approaches and collaboration with experts of different backgrounds for efficient use. Therefore, the Medical Faculty Mannheim of the Heidelberg University organized its research infrastructure in form of a Core Facility Pipeline, in which the Proteomics Core Facility (PCF) assumes a key position.Aim of the proposal is the technological enhancement of capabilities as well as the successive replacement of the main high throughput mass spectrometer at the PCF. The former shall reflect the technological development in the field in order to secure the means for high quality proteome analytics including dependable quantitation in support of scientific projects in life sciences. State of the art MS-platforms provide relative quantitative data for thousands of proteins in a single analysis per sample with improved specificity. Thus, they achieve improved depth of analysis and require less sample amount or reduced instrument time. Fundamental developments in data-independent acquisition (non-selective precursor analysis) achieve high data completeness and reproducibility with variation coefficients smaller than 20%. This improves the comparability of data and enables the discrimination of slight dynamic changes in protein expression. The high comparability provides essential prerequisites for digitalized biobanking. Finally, the main MS instrument of the PCF became less and less reliable. Therefore, the proposed instrument is intended to replace it step by step in the area of comparative analyses.The Medical Faculty Mannheim focuses on the four research areas Oncology, Vascular Biology and Medicine, Translational Neuroscience, and Medical Technology. Within the past years, these areas have been strengthened substantially by decisive actions of the faculty. In addition, novel and established cooperative research projects are pursued. As a result, the incremental number of scientific projects generates a higher demand for support by the PCF while asking for updated state of the art proteomics technologies. The basic and translational research of several medical and research departments representing multiple research groups benefit from the proposed instrumental enhancement of the PCF, which is reflected in the broad support of the grant application.

蛋白质组学包括分析生物样品的整个蛋白质补体，包括所有翻译后修饰。因此，它定义了功能基因组学的复杂表型。蛋白质组分析是现代生命科学中最具挑战性的，因为蛋白质的动态范围和可变性很大。先进的蛋白质组分析需要应用高度复杂的质谱分析方法，并与不同背景的专家合作，以实现高效使用。因此，海德堡大学医学院曼海姆以核心设施管道的形式组织了其研究基础设施，其中蛋白质组学核心设施（PCF）占据了关键位置。该提案的目的是提高技术能力，并连续更换PCF的主要高通量质谱仪。前者应反映该领域的技术发展，以确保高质量蛋白质组分析的手段，包括支持生命科学科学项目的可靠定量。最新技术水平的MS平台在每个样品的单次分析中提供了数千种蛋白质的相对定量数据，具有改进的特异性。因此，它们实现了改进的分析深度，并且需要更少的样品量或减少的仪器时间。数据独立采集（非选择性前体分析）的基本发展实现了高数据完整性和可重复性，变异系数小于20%。这提高了数据的可比性，并能够区分蛋白质表达的轻微动态变化。高可比性为数字化生物库提供了必要的先决条件。最后，PCF的主要MS仪器变得越来越不可靠。因此，拟议的仪器是为了取代它一步一步在比较分析领域。医学系曼海姆专注于四个研究领域肿瘤学，血管生物学和医学，转化神经科学，和医疗技术。在过去的几年里，这些领域已经大大加强了教师的果断行动。此外，新的和既定的合作研究项目进行。因此，科学项目数量的增加对PCF的支持产生了更高的需求，同时要求更新最先进的蛋白质组学技术。代表多个研究小组的几个医学和研究部门的基础和转化研究受益于PCF的拟议工具增强，这反映在赠款申请的广泛支持中。