Development of clean up technology for chlorinated organic compounds by using mirobes having high degradability

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1) Microbial population appeared at chlorinated ethene contaminated sites▲(most of TCE contaminants were transferred to cis-DCE)were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). Clones of 16S rRNA gene were not from disease-causing bacteria or opportunistic infectious disease causing bacteria. We concluded that the process does not accelerate development of any harmful microbes.2) Variations of DNA sequence of vinyl chloride reductase gene vcrA were analyzed at two TCE contaminated sites. All vcrA clones from different points at a site did not have the same DNA sequence. Slight changes in DNA sequences were found. This result suggest that a original vcrA gene has been changed with time at a site. Two sites surveyed showed almost the same trends. The gene of vcrA became a dominant dehalogenase gene among 7 cleaned up sites, showing that VcrA was a key enzyme for the complete dehalogenation.3) In summary, Dehalococcoides strains having vcrA gene was found to play an important role for the complete dehalogenation at TCE clean up sites. And the process did not increase any risks derived from microorganisms developed during the treatment period.