Development of clean up technology for chlorinated organic compounds by using mirobes having high degradability

利用高降解性微生物开发氯化有机化合物净化技术

• 批准号: 17206054
• 负责人: NAKAMURA Kanji
• 金额: $ 31.03万
• 依托单位: Tohoku Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17206054/
• 关键词: phonology; Game Theory; generative grammar; cognitive sciences; theory of communication; vcrA; 16SrRNA遺伝子; デハロコッコイデス; バイオオーグメンテーション; インバースPCR; bvcA; 塩素化エチレン; トリクロロエチレン; シスジクロロエチレン; 土壌浄化; リアルタイムPCR

1) Microbial population appeared at chlorinated ethene contaminated sites▲(most of TCE contaminants were transferred to cis-DCE)were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). Clones of 16S rRNA gene were not from disease-causing bacteria or opportunistic infectious disease causing bacteria. We concluded that the process does not accelerate development of any harmful microbes.2) Variations of DNA sequence of vinyl chloride reductase gene vcrA were analyzed at two TCE contaminated sites. All vcrA clones from different points at a site did not have the same DNA sequence. Slight changes in DNA sequences were found. This result suggest that a original vcrA gene has been changed with time at a site. Two sites surveyed showed almost the same trends. The gene of vcrA became a dominant dehalogenase gene among 7 cleaned up sites, showing that VcrA was a key enzyme for the complete dehalogenation.3) In summary, Dehalococcoides strains having vcrA gene was found to play an important role for the complete dehalogenation at TCE clean up sites. And the process did not increase any risks derived from microorganisms developed during the treatment period.

1）采用末端限制性片段长度多态性（T-RFLP）技术对氯化乙烯污染场地出现的微生物种群进行分析（大部分TCE污染物转移至cis-DCE）。 16S rRNA基因的克隆不是来自致病细菌或机会性传染病致病细菌。我们的结论是该过程不会加速任何有害微生物的生长。2)在两个TCE污染地点对氯乙烯还原酶基因vcrA的DNA序列的变异进行了分析。来自同一位点不同点的所有 vcrA 克隆并不具有相同的 DNA 序列。发现 DNA 序列发生轻微变化。该结果表明原始的vcrA基因在某个位点已随时间而改变。接受调查的两个网站显示出几乎相同的趋势。 vcrA基因成为7个清理位点中的优势脱卤酶基因，表明VcrA是完全脱卤的关键酶。3)综上所述，发现具有vcrA基因的Dehalococcoides菌株对TCE清理位点的完全脱卤发挥重要作用。而且该过程并没有增加治疗期间产生的微生物带来的任何风险。

项目成果

食品工場排水処理施設における活性汚泥の細菌群集構造と汚泥沈降性との関連性

食品厂废水处理设施活性污泥细菌群落结构与污泥沉降特性的关系

• 发表时间: 2007
• 期刊: 水環境学会誌 Vol.30
• 作者: 加藤 史子;飯泉 太郎;中村 寛治
• 通讯作者: 中村 寛治

Analysis of reductive dehalogenase genes functioning at biostimulation sites contaminated with chlorinated ethenes

受氯化乙烯污染的生物刺激位点功能的还原脱卤酶基因分析

• 发表时间: 2007
• 作者: R.Inada;Y.Fulcmnoto;Y.Mitsuno;Y.Nakamura;A.Oota;C.S.Li;P.X.Zhang;板井陽俊;K.Iwai;Kanji Nakamura
• 通讯作者: Kanji Nakamura

Relationship between bacterial community and sludge settleability in an activated sludge process for food industry

食品工业活性污泥工艺中细菌群落与污泥沉降性的关系

• 发表时间: 2007
• 期刊: Jour. of Japan Society on Water Environ Vol. 30
• 作者: Kato;F.;T. Iizumi;K. Nakamura
• 通讯作者: K. Nakamura

遺伝子からみた応用微生物学

从遗传学角度看应用微生物学

• 发表时间: 2006
• 作者: 中村寛治(共著;著者他多数)
• 通讯作者: 著者他多数)

塩素化エチレン類のバイオレメディエーションにおける微生物群集解析の応用

微生物群落分析在氯化乙烯生物修复中的应用

• 发表时间: 2005
• 期刊: 水環境学会誌 第28巻第8号
• 作者: 中村寛治;石田浩昭
• 通讯作者: 石田浩昭