Mechanism of High-Precision Molecular Computing and its Evaluation

本课题以实现高精度分子计算为目标，研究了基于DNA片段算法自组装的分子计算系统的几个方面。1)建立了一个能够解释DNA瓦片自组装过程细节的仿真模型。2)我们发现了一种新的现象，即衬底辅助自组装，与传统的自由溶液组装方法相比，我们可以在衬底表面制造出大的二维晶体，并且误差更小。我们成功地利用两种类型的T-motif tile进行了算法自组装，并将其应用于蛋白质阵列的制作。研制了C^<NVK>人工碱基，并验证了其在稳定DNA纳米结构和扩展DNA纳米结构多样性方面的作用。利用微流控装置逐步进行DNA自组装也得到了发展，并证明了荧光定量评价DNA瓦片纳米结构的可行性。

In this project, aiming at realization of high-precision molecular computing, we have investigated several aspects of molecular computing system based on algorithmic self-assembly of DNA tile. 1) A simulation model capable to explain details of self-assembly process of DNA tile has been developed. 2) We found a new phenomenon called substrate-assisted self-assembly, which enables us to make a large 2D crystal on substrate surface with less error compared with conventional assembly method in free solution. We have succeeded in algorithmic self-assembly using two types of T-motif tile and applied it to fabrication of protein array. Artificial base called C^<NVK> is developed, and its usefulness to stabilize DNA nanostructures and to extend the varieties of DNA nanostructure is verified. Stepwise DNA self-assembly by using micro-fluidic device is also developed, and feasibility of bulk evaluation of DNA tile nanostructure with fluorescent measurement has been shown by the method.