Mechanism of High-Precision Molecular Computing and its Evaluation

本课题以实现高精度分子计算为目标，对基于DNA瓦片算法自组装的分子计算系统进行了多方面的研究。1)建立了能够解释DNA碎片自组装过程细节的模拟模型。2)我们发现了衬底辅助自组装的一种新现象，与传统的自由溶液组装方法相比，这种方法可以在衬底表面制造出大尺寸的二维晶体，并且误差较小。我们已经成功地利用两种类型的T基序瓦片进行了算法自组装，并将其应用于蛋白质阵列的制作。开发了一种名为C^&lt；NVK&GT；的人工碱基，并验证了其在稳定DNA纳米结构和扩展DNA纳米结构多样性方面的有效性。利用微流控装置实现了DNA的逐步自组装，并用该方法对DNA碎片纳米结构进行了整体荧光检测的可行性。

In this project, aiming at realization of high-precision molecular computing, we have investigated several aspects of molecular computing system based on algorithmic self-assembly of DNA tile. 1) A simulation model capable to explain details of self-assembly process of DNA tile has been developed. 2) We found a new phenomenon called substrate-assisted self-assembly, which enables us to make a large 2D crystal on substrate surface with less error compared with conventional assembly method in free solution. We have succeeded in algorithmic self-assembly using two types of T-motif tile and applied it to fabrication of protein array. Artificial base called C^<NVK> is developed, and its usefulness to stabilize DNA nanostructures and to extend the varieties of DNA nanostructure is verified. Stepwise DNA self-assembly by using micro-fluidic device is also developed, and feasibility of bulk evaluation of DNA tile nanostructure with fluorescent measurement has been shown by the method.