Highly quantitative gene analysis based on affinity capillary electrophoresis using DNA conjugates

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To quantitatively detect point gene mutation, we have developed an affinity capillary electrophoresis using a block copolymer probe composed of a synthetic polymer and a single-stranded DNA. During electrophoresis of a mixture of the target and nontarget DNA, the affinity probe binds selectively to the target DNA and thus decreases the electrophoretic mobility by exerting a large amount of hydrodynamic friction. Moreover, this study demonstrated that(1) the peak resolution was improved in the presence of electroosmotic flow and(2) a block copolymer probe containing peptide nucleic acid sequence-selectively bound to secondary-structured target DNA.