Constructive research for understanding the effects of amyloid β to neuronal function.

阿尔茨海默病（Alzheimer's disease，AD）是以β淀粉样肽（amyloid β peptide，Aβ）聚集为特征的疾病。本研究的目的是通过多电极阵列（MEA）记录的神经元细胞电活动的慢性监测来研究A β毒性对胚胎大鼠海马神经元的影响，以及保护神经元免受Aβ毒性的分子。应用Aβ对培养的胚胎大鼠海马神经元的自发放电有抑制作用，作用24h后自发放电开始抑制，72h后自发放电消失。当Aβ与黑种草油中的活性成分百里醌（TQ）同时作用时，百里醌在作用后34h可减轻Aβ的毒性作用。提示TQ对Aβ损伤海马细胞具有保护作用。

Alzheimer's disease (AD) is characterized by the aggregation of amyloid β peptide (Aβ). The aim of this study is to investigate the effect of Amyloid Beta toxicity in embryonic rat hippocampus through chronic monitoring of neuronal cell electrical activity using multielectrode array (MEA) recordings, and the molecules protecting neurons from Aβ toxicity. Applying Aβ to cultured embryonic rat hippocampal neurons has an inhibitory effect on spontaneous activity started 24h after applying and the firing disappeared at 72h. When thymoquinone (TQ) active constituent of Nigella Sativa Oil and Aβ were simultaneously applied, thymoquinone reduces the deleterious effect of Aβ at 34h after applying. This results indicated that TQ have the potential protecting hippocampus cells from Aβ toxicity.