Reliability evaluation of fluorescence probe method for molecular diffusion measurement in lipid bilayer by atomic force microscopy

在本项目中，开发了一种原子力显微镜（AFM）仪器，以评估荧光探针方法用于脂质双分子层分子扩散测量的可靠性。本项目研制的样品架在相同条件下可用于分子分辨率原子力显微镜成像和荧光测量。由于所开发的AFM仪器允许我们比较两种方法获得的分子扩散数据，因此该能力应有助于评估荧光探针方法用于分子扩散测量的可靠性。

In this project, an atomic force microscopy (AFM) instrument was developed to evaluate the reliability of fluorescence probe methods for molecular diffusion measurements in lipid bilayers. A sample holder developed in this project is usable in both the molecular-resolution AFM imaging and fluorescence measurements under the same condition. As the developed AFM instrument allows us to compare the data of molecular diffusion obtained by both methods, the capability should be useful for the reliability evaluation of fluorescence probe methods for molecular diffusion measurements.