Reliability evaluation of fluorescence probe method for molecular diffusion measurement in lipid bilayer by atomic force microscopy

本计画利用原子力显微镜（AFM）来评估萤光探针法测量脂双层分子扩散的可靠性。本计画所开发的样品保持器可在相同条件下同时用于分子分辨AFM成像与萤光量测。由于所开发的AFM仪器允许我们比较通过两种方法获得的分子扩散的数据，该能力应该是有用的分子扩散测量的荧光探针方法的可靠性评估。

In this project, an atomic force microscopy (AFM) instrument was developed to evaluate the reliability of fluorescence probe methods for molecular diffusion measurements in lipid bilayers. A sample holder developed in this project is usable in both the molecular-resolution AFM imaging and fluorescence measurements under the same condition. As the developed AFM instrument allows us to compare the data of molecular diffusion obtained by both methods, the capability should be useful for the reliability evaluation of fluorescence probe methods for molecular diffusion measurements.