Development of a platform for a novel proteome analysis in a living cell utilizing proteoinjection

我们已经建立了一种新的蛋白质组分析的基本技术，该技术通过分析直接将蛋白质引入细胞（蛋白质注射）引起的细胞反应来评估活细胞中蛋白质的功能卷。我们已经成功开发了一种技术，可以将具有功能的蛋白质引入单目标活ES细胞。此外，研究发现，在探针DNA中插入转录因子特异性序列可以提高活ES细胞中报告基因的表达效率。因此，我们提出了一种新的方法来评估活ES细胞内转录因子的功能卷，这取决于探针表达效率的提高。

We have established basic technology for a novel proteome analysis in which the functional rolls of proteins in a living cell are evaluated by the analysis of cellular response induced by the direct introduction of the proteins into a cell (proteoinjection). We have successfully developed a technology which enables us to introduce proteins with functions into a single-target-living ES cell. Moreover, it has been found that the simple insertion of a transcriptional factor specific sequence into a probe DNA enhances the efficiency of the expression of a reporter in a living ES cell. Therefore it has been suggested a new method to evaluate the functional rolls of transcriptional factors inside living ES cells depending on the enhancement of the probe expression efficiency.