Development of a platform for a novel proteome analysis in a living cell utilizing proteoinjection

我们已经建立了一种新的蛋白质组分析的基本技术，其中蛋白质在活细胞中的功能卷通过分析由蛋白质直接引入细胞（蛋白质注射）诱导的细胞反应来评估。我们成功开发了一种技术，使我们能够将具有功能的蛋白质引入单靶点活体ES细胞中。此外，已经发现，将转录因子特异性序列简单插入探针DNA中可提高报告基因在活ES细胞中表达的效率。因此，我们提出了一种新的方法来评估活的ES细胞内的转录因子的功能卷依赖于探针表达效率的提高。

We have established basic technology for a novel proteome analysis in which the functional rolls of proteins in a living cell are evaluated by the analysis of cellular response induced by the direct introduction of the proteins into a cell (proteoinjection). We have successfully developed a technology which enables us to introduce proteins with functions into a single-target-living ES cell. Moreover, it has been found that the simple insertion of a transcriptional factor specific sequence into a probe DNA enhances the efficiency of the expression of a reporter in a living ES cell. Therefore it has been suggested a new method to evaluate the functional rolls of transcriptional factors inside living ES cells depending on the enhancement of the probe expression efficiency.