Development of fluorescent strain sensor and imaging system

为了检测外力作用下活细胞中蛋白质的构象变化，对基于GFP的荧光应变传感器进行了改进和评价。此外，为了建立适合传感器的成像系统，我从理论上估计了比率传感器的比率值的最大误差，通过线性光谱解混，这是基于最小二乘回归方法。该估计将提供一个有用的标准，以验证由比率传感器的光谱成像所获得的结果。

To detect external force-induced conformational changes of proteins in living cells, I improved and evaluated the GFP-based fluorescent strain sensor. Also, toward the establishment of an imaging system suitable for the sensor, I theoretically estimated the maximum errors in the ratio values of ratiometric sensors, obtained by linear spectral unmixing, which is based on the least squares regression method. The estimation would provide an useful criterion to validate the results obtained by spectral imaging of ratiometric sensors.