Elucidating signaling components under low potassium conditions

将农杆菌文库转化拟南芥AtHAK5启动子：：荧光素酶基因植株。对这些带有Fox文库的AtHAK5启动子：：荧光素酶植株进行了筛选，获得了200多株在全营养条件下具有较高荧光素酶活性的候选转基因植株。其中，对15个品系进行了分析，以确定哪个基因是高表达的，并对一些候选基因的表达进行了分析，以应对营养缺乏。获得了WRKY Tf的过表达植株和敲除植株。过量表达植株表现出对缺钾条件的耐受性增强。

Arabidopsis FOX (Full-length cDNA over-expressor genes) agrobacterium librarys were transformed into established AtHAK5 promoter::luciferase Arabidopsis plants. These AtHAK5 promoter::luciferase plants with FOX library have been screened and more than 200 candidate transgenic plants that have higher luciferase activity under full nutrient condition have been isolated. Among them, fifteen lines were analyzed to determine which gene is overexpressed, and some candidates were analyzed for their expression in response to nutrient deficiency. The overexpression plants and knockout plants of WRKY TF were obtained. The overexpression plants showed increased tolerance to potassium deficient condition.