Assessment of antigen processing and presentation of CD40 activated B cells

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It has been demonstrated that CD40-activated B (CD40-B) cells are potent antigen presenting cells that can be expanded significantly ex vivo.Several methods to antigen load CD40-Bs to generate viral or tumor-antigen specific CD8+ T cells have been identified. However, it is unclear how CD40-Bs take up and process exogenous antigen in an B cell receptor independent manner to stimulate CD4+ T cells. CD4+ T cells specific for multiple epitopes were successfully generated in stimulation with CD40-B cells pulsed with recombinant protein. In addition, CD40-Bs were able to be easily transfected with in vitro transcribed-mRNA. CD40-B cells are thought to be useful in cellular therapy.