In Vitro Cytotoxicity Model of Particulate Matter on Human Red Blood

我们的研究结果表明，二氧化钛（TiO 2）颗粒在溶液中的悬浮方法会影响颗粒对红细胞（RBC）或任何其他细胞系的影响，因此必须采用精确和标准的分散方法。我们以前的实验和目前的结果表明，任何形式的TiO_2都会引起溶血。虽然颗粒大小是由TiO 2诱导的溶血水平的重要决定因素，但颗粒类型或多晶型起着更重要的作用。我们通过检查颗粒对谷胱甘肽氧化和脂质过氧化的影响，红细胞膜的初步电泳实验表明，TiO_2对红细胞膜的电泳有一定的影响。在低分子量蛋白上的2个颗粒，其被认为是Cofilin。TiO 2颗粒暴露于A549肺上皮细胞后，细胞凋亡呈剂量依赖性，IL-8 mRNA和IL-8蛋白的释放略有增加，但对NF-κ B DNA无影响。

Our results suggest that the method of suspension of titanium dioxide (TiO2) particles in solution influences the effects of the particles on red blood cells (RBCs) or any other cell line, therefore precise and standard dispersion methods must be employed. Our previous experiments and the current results demonstrate that any form of TiO_2 causes hemolysis. Although particle size is an important determinant of the level of hemolysis induced by TiO2, the particle type or polymorph plays a more significant role.We investigated the involvement of oxidative stress in TiO2-induced hemolysis by examining the effects of particles on glutathione oxidation and lipid peroxidation, and the results were inconclusive.Preliminary electrophoresis experiments with RBC membranes showed a probable effect of the TiO_2 particles on a low molecular weight protein, which was considered to be Cofilin. However, poor reproducibility of these results hindered our confirmation of this finding.TiO2 particles exposed to A549 lung epithelial cells caused dose-dependent apoptosis, slight increase in the release of IL-8 mRNA and IL-8 protein, but no effects on NF-kB DNA.