Cell-type and activation-specific labelling of different leukocyte populations in vivo by bioorthogonal tagging (A01)

体内不同白细胞群的成像是了解其动力学和在炎症中作用的关键。为了克服目前的局限性，我们将开发新的方法，在更长的时间内标记免疫细胞，即，从炎症开始到消退。我们将建立一个灵活的模块化平台，适用于不同的模式，如荧光，荧光或光声成像。ER-HoxB 8细胞将用抗体片段的自标记酶或表位离体进行遗传标记。在分化和再注射后，将通过施用标签特异性的功能化标记来实现体内标记，以选择性地使皮肤肉芽肿小鼠模型中的相应细胞群可视化。

Imaging of different leukocyte populations in vivo is key to understand their dynamics and role in inflammation. To overcome current limitations, we will develop new methods to label immune cells within a longer period of time, i.e., from inflammation initiation to resolution. We will establish a flexible and modular platform suitable for different modalities, such as fluorescence, scintigraphic or photoacoustic imaging. ER-HoxB8 cells will be genetically tagged ex vivo with self-labelling enzymes or epitopes for antibody fragments. After differentiation and re-injection, in vivo labelling will be achieved by administering tag-specific, functionalized labels to selectively visualize the respective cell population in a cutaneous granuloma mouse model.