Development of the cytoplasmic molecular delivery by the photodynamic treatment inducing the mild oxidation of the plasma membrane

通过光动力处理诱导质膜轻度氧化来发展细胞质分子递送

• 批准号: 22800059
• 负责人: MIYAMOTO Yuichi
• 金额: $ 1.78万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22800059/
• 关键词: 生物・生体工学; ナノバイオ; 細胞・組織; 光線力学的治療; ナイバイオ

Photodynamic therapy(PDT) established as a curative treatment for the cancer has acquired anticancer efficacy by producing reactive oxygen species on theplasma membrane orthe cytoplasmic organelle. In this study, I employed PDT inducing no remarkable cell injury and investigate whether the PDT(1) can deliverthe macromoleculeto the cell,(2) can enhance the cytotoxic effect of the anticancer drug. As the results, the PDT would transfer a molecule of 10 kDa or less through the plasma membrane of the HeLa cells and enhance the cytotoxic effects of Bleomycin.

光动力学疗法（PDT）是一种通过在细胞质膜或细胞器上产生活性氧来治疗肿瘤的方法。在本研究中，我采用PDT诱导无明显细胞损伤，并研究PDT是否（1）能将大分子递送到细胞，（2）能增强抗癌药物的细胞毒效应。结果，PDT将通过HeLa细胞的质膜转移10 kDa或更小的分子，并增强博来霉素的细胞毒性作用。

项目成果

Effect of 630-nm pulsed laser irradiation of HeLa cells in Photofrin-mediated photodynamic therapy

630 nm 脉冲激光照射 HeLa 细胞在 Photofrin 介导的光动力治疗中的效果

• 发表时间: 2011
• 期刊: Laser Therapy
• 作者: Yuichi Miyamoto;Daisuke Nishikiori;Fumika Hagino;Masayoshi Wakita;Ichiro Tanabe;Masahiro Toida
• 通讯作者: Masahiro Toida