Investigation of the selective ion transport system of mammary epithelial cells

乳腺上皮细胞选择性离子转运系统的研究

• 批准号: 22880003
• 负责人: KAMIKAWA Akihiro
• 金额: $ 1.86万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22880003/
• 关键词: 乳腺; イオンチャネル; 上皮細胞; 電気生理学; 発達

It has been suggested that the selective ion transport of mammary epithelial cells play an important role in lactation. However, active ion channels in mammary secretory cells are unknown. To reveal the mechanisms of lactation, we investigated the active ion channels in mammary secretory cells. A whole-cell patch clamp technique was applied to mammary epithelial cells (MEC) freshly isolated from mammary glands of lactating mice. In this study, we found the functional expression of inwardly rectifying potassium (Kir) channels in lactating MEC.

有人提出，乳腺上皮细胞的选择性离子转运在哺乳中发挥重要作用。然而，乳腺分泌细胞中的活性离子通道尚不清楚。为了揭示泌乳机制，我们研究了乳腺分泌细胞中的活性离子通道。将全细胞膜片钳技术应用于从哺乳小鼠乳腺中新鲜分离的乳腺上皮细胞（MEC）。在这项研究中，我们发现了泌乳期 MEC 中内向整流钾 (Kir) 通道的功能表达。