Role of nutrient sensor AMPK on regulation of myogenic inhibitory factor myostatin.

从牛骨骼肌建立了能有效分化为肌管的原代成肌细胞。成肌细胞在增殖过程中表达成肌细胞标志物Myf5，在分化过程中表达分化标志物Mygenin。这些细胞在低糖条件下被抑制分化和MSTN的表达，从而激活AMPK。而MSTN受体的表达无明显变化。AMPK激动剂AICAR也能抑制MSTN在肌管中的分化和表达。AICAR可阻断MSTN信号转导拮抗剂的作用。这些结果表明，AMPK对MSTN的作用有调节作用。

Primary myoblasts that can efficiently differentiate to myotube was established from bovine skeletal muscle. The myoblasts was expressed myoblast marker Myf5 during proliferation and differentiation marker myogenin during differentiation. These cells were inhibited differentiation and MSTN expression under low glucose condition, which activates AMPK. However expression of MSTN receptor was not changed. AMPK activator AICAR also inhibited differentiation and expression of MSTN in myotubes. The effect of antagonist of MSTN signaling was abolished by AICAR. These results suggest that AMPK regulates the action of MSTN.