Creation of polyclonal antibody of secreted protein SEPINE2, which is expressed in testicular cancer cell line with high metastatic potential. How does it work in testicular cancer metastasis?

创建分泌蛋白 SEPINE2 的多克隆抗体，该蛋白在具有高转移潜力的睾丸癌细胞系中表达。

• 批准号: 22890108
• 负责人: NAGAHARA Akira
• 金额: $ 1.96万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890108/
• 关键词: 精巣腫瘍; 転移; SERPINE2; SEPINE2

We tried to create a polyclonal antibody of secreted protein SERPINE2 that we had reported before and to elucidate how it work in testicular cancer metastasis. Although we established stable SERPINE-expressed testicular cancer cell line, we couldn' t get evidence that SERPINE2 was metastatic promoter in vivo and mRNA expression analysis in clinical samples. And we regretted to create the antibody of SERPINE2.

我们试图创建一种分泌蛋白SERPINE2的多克隆抗体，我们之前已经报道过，并阐明它如何在睾丸癌转移中起作用。虽然我们建立了稳定的表达SERPINE2的睾丸癌细胞系，但我们在体内和临床样品的mRNA表达分析中没有得到SERPINE2是转移启动子的证据。我们很后悔创造了SERPINE2的抗体。