Photodynamic therapy : A new treatment for rheumatoid arthritis

光动力疗法：类风湿性关节炎的新疗法

• 批准号: 22890214
• 负责人: KAGEYAMA Yasunori
• 金额: $ 1.46万
• 依托单位: Hamamatsu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890214/
• 关键词: 関節リウマチ; 光線力学療法; PDT; 5-aminolevurinic acid

Because the main focus of rheumatoid arthritis(RA) is synovial membranes, synovectomy has been carried out for the treatment of RA. Now we performed basic research to aim to make application of photodynamic therapy(PDT), which is relatively few damage to the human body, to synovectomy in patients with RA. We performed PDT to cultured synovial cells from RA using ATX-S10(Na), Photofrin, and 5-aminolevurinic acid(5-ALA) as photosensitizers. The PDT effect of all photosensitizers was obtained. Type II collagen-induced arthritis of mice is an animal model of RA. In this model, the fluorescence of the joints under the excitation of 405 nm laser was measured at 2-3 hours after administration of Photofrin or 5-ALA. Although the fluorescence was detected in the all joints, the significant difference in fluorescence intensity between in arthritic joints and in non-arthritic joints was not seen. The application of PDT by using Photofrin and 5-ALA to synovectomy in the experimental animal model has been considered to have some problems to solve.

由于类风湿性关节炎（RA）的主要焦点是滑膜，因此滑膜切除术已被用于治疗RA。目前我们正在进行基础研究，旨在将对人体损伤较小的光动力疗法（PDT）应用于RA患者的滑膜切除术。我们使用ATX-S10（Na）、Photofrin和5-氨基乙酰尿酸（5-ALA）作为光敏剂对RA培养的滑膜细胞进行PDT。得到了所有光敏剂的PDT效应。小鼠II型胶原性关节炎是类风湿性关节炎的动物模型。在本模型中，在给予Photofrin或5-ALA 2-3小时后，测量关节在405 nm激光激发下的荧光。虽然在所有关节中都检测到荧光，但在关节炎关节和非关节炎关节中没有观察到荧光强度的显著差异。在实验动物模型中，利用Photofrin和5-ALA进行PDT在滑膜切除术中的应用一直被认为有一些问题需要解决。

项目成果

Novel 5-ALA-PDD/PDT combined therapyモデルの開発研究

新型5-ALA-PDD/PDT联合治疗模式的开发研究

• 发表时间: 2011
• 作者: 吉田孝人;河野栄治;金田雅充;井上克司;石井拓也;影山康徳;福司康子;寺川進
• 通讯作者: 寺川進

関節リウマチ培養滑膜細胞に対する光線力学療法(PDT)の効果

光动力疗法（PDT）对培养类风湿性关节炎滑膜细胞的影响

• 发表时间: 2011
• 作者: 影山康徳;鳥養栄治;小林勇人
• 通讯作者: 小林勇人

担腫瘍ヌードマウスを用いたALA-PDTのcombined therapyモデルの研究

荷瘤裸鼠ALA-PDT联合治疗模型研究

• 发表时间: 2011
• 作者: 吉田孝人;河野栄治;金田雅充;井上克司;影山康徳;福司康子;寺川進
• 通讯作者: 寺川進