The assembly of an animal experimental model system for cell transplantation therapy using allogeneic stem cells with severe cardiac failure.

我们建立了猪羊膜、胎盘和脐带来源的间充质细胞。它们的间充质细胞表达间充质干细胞标志物，就像人类细胞一样。将转染增强型绿色荧光蛋白（EGFP）的细胞直接移植于猪慢性心肌缺血模型的损伤心肌内。在移植后4周，在免疫活性宿主心脏中鉴定了注射的表达EGFP的同种异体AMSC，而不使用任何免疫抑制剂。免疫组化显示EGFP与心肌肌钙蛋白T和心肌肌钙蛋白I共定位。

We established porcine amniotic membrane, placenta and umbilical cord-derived mesenchymal cells. Their mesenchymal cells were expressed mesencymal stem cell markers, just as human cells. The cells transduced with enhanced green fluorescent protein(EGFP) were directly transplanted in injured myocardium on porcine chronic myocardial ischemic model. Injected allogeneic EGFP-expressing AMSCs were identified in the immunocompetent host heart without the use of any immunosuppressants at 4 weeks after transplantation. Immunohistochemistry revealed that EGFP colocalized with cardiac troponin T and cardiac troponin I.