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Control of gibberellin signaling, gibberellin sensing and interplay of gibberellin with sucrose

赤霉素信号传导、赤霉素传感以及赤霉素与蔗糖相互作用的控制

基本信息

批准号：
22H00360
负责人：
Frommer Wolf
金额：
$ 27.21万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H00360/
关键词：
Phytohormone Gibberelllic acid Sugar SWEETs Transporter Gibberellic acid

项目摘要

Gibberellic acid (GA) and sugars can have synergistic effects on plant growth and yield. Surprisingly, members of the SWEET transporter family, which are responsible for sucrose transporter, have also been found to transport GA. This dual specificity of SWEETs for sugars and GA provides a potential nexus to understand the interactions between these two compounds. We here develop approaches to dissect the contribution of sugars and GA and lay the basis for engineering improved plants. To understand how sucrose and GA bind to SWEET, we performed computational simulations, specifically molecular dynamics simulation and docking simulation between SWEET13 and sucrose as well as GA based on their structures. Through these simulations, we identified amino acids in SWEET13 that recognize sucrose and GA. Subsequently, using cell cultures, we confirmed that these amino acids were necessary to recognize sucrose and GA when they bind to SWEET13. These findings have allowed us to manipulate the selectivity of SWEET for two substrates, sucrose and GA.

赤霉酸（GA）和糖可以对植物生长和产量产生协同作用。令人惊讶的是，负责蔗糖转运蛋白的 SWEET 转运蛋白家族的成员也被发现可以转运 GA。 SWEET 对糖和 GA 的双重特异性为理解这两种化合物之间的相互作用提供了潜在的联系。我们在这里开发方法来剖析糖和 GA 的贡献，并为工程改良植物奠定基础。为了了解蔗糖和GA如何与SWEET结合，我们进行了计算模拟，特别是分子动力学模拟以及SWEET13与蔗糖以及GA基于其结构的对接模拟。通过这些模拟，我们鉴定了 SWEET13 中识别蔗糖和 GA 的氨基酸。随后，使用细胞培养物，我们证实这些氨基酸在与 SWEET13 结合时是识别蔗糖和 GA 所必需的。这些发现使我们能够控制 SWEET 对两种底物（蔗糖和 GA）的选择性。