Functionalized capillary device for mass spectrometry-based subcellular lipid profiling

用于基于质谱的亚细胞脂质分析的功能化毛细管装置

• 批准号: 22K20560
• 负责人: LIU CHENCHEN
• 金额: $ 1.83万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20560/
• 关键词: single cell analysis; subcellular analysis; nano ESI; capillary needle; mass spectrometry; cell membrane lipid; lipid raft; lipid profiling; Single cell analysis; Electrophoresis; Mass spectroscopy; Molecularly imprinting; Lipid profiling

The objective of this project is to develop a capillary device that can perform subcellular lipid profiling. This device will allow for minute sampling, lipid purification and separation, and nano electrospray ionization for mass spectrometry (MS) analysis. Our preliminary tests have shown that a lab-made capillary device can be used for standard lipid MS detection. The device successfully achieved minute sampling and nano electrospray ionization, which have been integrated into a single capillary device. Building on this promising outcome, we plan to integrate the function for lipid purification and separation into the capillary device as well.

该项目的目标是开发一种可以进行亚细胞脂质分析的毛细血管装置。该设备将允许分钟采样，脂质纯化和分离，以及用于质谱（MS）分析的纳米电喷雾电离。我们的初步试验表明，实验室自制的毛细管装置可用于标准脂质MS检测。该装置成功地实现了微小的采样和纳米电喷雾离子化，已集成到一个单一的毛细管装置。基于这一有希望的结果，我们计划将脂质纯化和分离的功能也集成到毛细管装置中。

项目成果

New studies on poly(ethylene glycol)-based hydrogels in electrophoresis

聚乙二醇水凝胶电泳新研究

• 发表时间: 2022
• 作者: Kusada Kohei;Mukoyoshi Megumi;Wu Dongshuang;Kitagawa Hiroshi;Chenchen Liu
• 通讯作者: Chenchen Liu