Directing Human Induced Pluripotent Stem Cell-Derived Embryonic-like Organoid with 3D Biomimetic Cryogel Mechanical Microenvironment for Neural Induction.

利用 3D 仿生冷冻凝胶机械微环境指导人类诱导多能干细胞衍生的胚胎样器官进行神经诱导。

• 批准号: 22K20642
• 负责人: Vadivelu RajaKumar
• 金额: $ 1.83万
• 依托单位: Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20642/
• 关键词: hIPSCs; Gastruloid; Cryogel; Mechanobiology; Neural Induction; Neuromesoderm; organogenesis; pluripotent; Embryonic Organoid; Neuromesodermal

We aim to investigate how using cryogel sandwich model by embedding cell clusters with layer-by-layer cryogel could enhance mechanical constraints. First, we studied cryogel`s topology by using Hirox, and SEM microscope analysis revealed that cryogel surface topology consists of voids that appear as hill-valley-like structures composed of pockets like void space which can trap microbeads. Thus, cryogel facilitates spontaneous cell cohesion, leading to spherical-shaped aggregates within 24h. After cryogel embedding the aggregates acquire a teardrop-like shape. Eventually, on day 3 these aggregates showed up ovoid shape which resembles a symmetrical breaking process. Day 4 showed polarized aggregates with the posterior axis.

我们的目的是研究如何使用冷冻凝胶三明治模型，通过嵌入细胞团与逐层冷冻凝胶可以增强机械约束。首先，我们使用Hirox研究了晶胶的拓扑结构，SEM显微镜分析显示晶胶表面拓扑结构由空隙组成，这些空隙呈山谷状结构，由可以捕获微珠的空隙空间等口袋组成。因此，冷冻凝胶促进自发细胞凝聚，导致在24小时内的球形聚集体。冷冻凝胶包埋后，聚集体获得泪滴状形状。最终，在第3天，这些聚集体显示出类似于对称断裂过程的卵形形状。第4天显示具有后轴的极化聚集体。

项目成果

Cryogel mechanical interface for directing Human Induced Pluripotent Stem Cells (hIPSCs) derived gastruloid-like organoids with organized neuromesodermal integration

Cryogel 机械接口，用于引导人类诱导多能干细胞 (hIPSC) 衍生的类原肠胚样器官与有组织的神经中胚层整合

• 发表时间: 2023
• 作者: 川口 隆之;Alexis HARDAY;Amandine TOUZEAU;Sandra DUHARCOURT;中山 潤一;Raja Kumar Vadivelu
• 通讯作者: Raja Kumar Vadivelu