Directing Human Induced Pluripotent Stem Cell-Derived Embryonic-like Organoid with 3D Biomimetic Cryogel Mechanical Microenvironment for Neural Induction.

我们的目的是研究如何使用冷冻凝胶三明治模型，通过嵌入细胞团与逐层冷冻凝胶可以增强机械约束。首先，我们使用Hirox研究了晶胶的拓扑结构，SEM显微镜分析显示，晶胶表面拓扑结构由空隙组成，这些空隙看起来像是由口袋状空隙空间组成的山谷状结构，可以捕获微珠。因此，冷冻凝胶促进自发细胞凝聚，导致在24小时内的球形聚集体。冷冻凝胶包埋后，聚集体获得泪滴状形状。最终，在第3天，这些聚集体显示出类似于对称断裂过程的卵形形状。第4天显示具有后轴的极化聚集体。

We aim to investigate how using cryogel sandwich model by embedding cell clusters with layer-by-layer cryogel could enhance mechanical constraints. First, we studied cryogel`s topology by using Hirox, and SEM microscope analysis revealed that cryogel surface topology consists of voids that appear as hill-valley-like structures composed of pockets like void space which can trap microbeads. Thus, cryogel facilitates spontaneous cell cohesion, leading to spherical-shaped aggregates within 24h. After cryogel embedding the aggregates acquire a teardrop-like shape. Eventually, on day 3 these aggregates showed up ovoid shape which resembles a symmetrical breaking process. Day 4 showed polarized aggregates with the posterior axis.