Directing Human Induced Pluripotent Stem Cell-Derived Embryonic-like Organoid with 3D Biomimetic Cryogel Mechanical Microenvironment for Neural Induction.

我们的目标是研究如何使用冷冻三明治模型通过将细胞团逐层嵌入冷冻胶来增强机械约束。首先，我们用Hirox研究了冷冻胶的拓扑结构，扫描电子显微镜分析表明，冷冻胶的表面拓扑结构是由山谷状的结构组成的，这些空洞是由空穴状的空间组成的，这些空穴可以捕获微珠。因此，冷冻促进了自发的细胞凝聚力，导致在24小时内形成球形聚集体。冰激凌包埋后，聚集体呈泪滴状。最后，在第3天，这些聚集体呈现卵圆形，类似于对称的破碎过程。第4天后轴线呈极化集合体。

We aim to investigate how using cryogel sandwich model by embedding cell clusters with layer-by-layer cryogel could enhance mechanical constraints. First, we studied cryogel`s topology by using Hirox, and SEM microscope analysis revealed that cryogel surface topology consists of voids that appear as hill-valley-like structures composed of pockets like void space which can trap microbeads. Thus, cryogel facilitates spontaneous cell cohesion, leading to spherical-shaped aggregates within 24h. After cryogel embedding the aggregates acquire a teardrop-like shape. Eventually, on day 3 these aggregates showed up ovoid shape which resembles a symmetrical breaking process. Day 4 showed polarized aggregates with the posterior axis.