Exploring the exosome-nanosome combinatorial platform as a novel, universal, and ideal nanoparticle-based drug delivery system

在研究实施的第一个财政年度，生产和分离已经优化，以产生大量的外泌体。这部分对于“纳米小体”的结合很重要，因为要产生的“外泌体-纳米小体”平台的数量取决于所产生的外泌体。在喂食和饥饿细胞之间产生的外泌体的数量也被确定为不同的。一些外泌体蛋白在饥饿状态下下调。这些都是非常重要的，因为“外泌体-纳米小体”在不同细胞/器官上的结合也会受到显著影响。平台的构建显示了“纳米小体”的结合和优化（尺寸，配体组成和总体电荷）似乎是必要的。

During the first fiscal year of research implementation, production and isolation have been optimized to yield high number of exosomes. This part is important for the incorporation of "nanosome" as the number of "exosome-nanosome" platform to be produced is dependent on the produced exosomes. The amount of exosome produced between fed and starved cells were also determined to be different. Some exosome proteins are downregulated in the starved state. These are significantly important as the "exosome-nanosome" incorporation on different cells/organs can also be impacted significantly.The construction of the platform showed limitations on the incorporation of the "nanosome" and optimization (sizes, ligand composition, and the overall charge) seemed to be necessary.