Genome - wide analysis for PRC1 - bound RNA molecules .

多梳抑制复合物（PRC 1和PRC 2）是一类重要的蛋白质复合物，通过对组蛋白进行翻译后修饰实现表观遗传基因沉默。PRC 1和PRC 2被认为是通过与RNA分子结合来实现其转录沉默功能的。使用RNA免疫沉淀结合下一代DNA测序仪（RIP-seq）针对Ring 1B蛋白（PRC 1的一个组成部分），我们最近以全基因组的方式鉴定了这些RNA分子。令人惊讶的是，PRC 1的许多组分的转录物包括在Ring 1B结合的RNA组分中。这些结果表明，PRC组分的量可能是由PRC本身控制的，提供了一种微调机制来维持PRC的恒定表达水平。

Polycomb repressive complexes (PRC1 and PRC2) are important protein complexes, which carry out epigenetic gene silencing by adding post-translational modifications to Histone proteins. PRC1 and PRC2 have been proposed to execute their transcriptional silencing function through binding with RNA molecules. Using RNA immunoprecipitation combined with next-generation DNA sequencer (RIP-seq) against Ring1B protein, a component of PRC1, we have recently identified those RNA molecules in a genome-wide manner. Surprisingly, transcripts of many components of PRC1 are included in the Ring1B-bound RNA fractions. These results suggest that the amount of PRC components may be controlled by PRCs themselves, providing a fine-tuning mechanism to maintain constant expression level of PRCs.