Construction of artificial synapse liposome array using the vesicular transport liposome model

本研究利用脉冲射流技术从平面脂双层膜制备非对称细胞大小的脂囊泡。首先，使用改进的双孔装置稳定地形成细胞大小的囊泡（在1天内直径为5 μm），其中在威尔斯孔之间安装具有150 μ m孔径的分离器。此外，使用该装置在每个瓣叶上成功形成磷脂酰丝氨酸脂质的不对称囊泡。非对称细胞大小的脂质囊泡将为人工细胞研究模型如脂膜与蛋白质的相互作用创造新的机会。

This study conducted the preparation of asymmetric cell-sized lipid vesicles from a planar lipid bilayer using pulsed jet flow. First, cell-sized vesicles (diameter of 5 μm in over 1 day) were stably formed using an improved double-well device wherein a separator with a 150-μm-hole diameter, was mounted between the wells. Moreover, asymmetric vesicles of phosphatidylserine lipids were successfully formed on each leaflet using this device. The asymmetric cell-sized lipid vesicles will create new opportunities for artificial cell research model such as the interactions between lipid membranes and proteins.