Analysis of the cell division patterning during plant early embryogenesis by developing the optical manipulation techniques

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Multicellular animals and plants develop from the single-celled zygote to form the mature embryo. During embryogenesis, the zygote follows a pattern of cell division to form the body plan of the embryo. In this study, I performed live-cell imaging using in vitroovule culture and the fluorescent marker lines in Arabidopsis thaliana. The data of live-cell imaging indicated that the timings of cell division are not strictly synchronized even in 4-cell embryo stage. Moreover, I established the gene induction system in a single-cell level in Arabidopsis embryo using the optical manipulation technique.