he mechanisms of endocytic transport for activate receptor in budding yeast

为了阐明激活受体如何被募集到网格蛋白包被坑中的机制，我们筛选酵母基因组文库，鉴定出14-3-3蛋白的同源物Bmh2p作为酵母GPCR的结合蛋白。此外，我们用GFP标记了13个人GPCR，并成功地在酵母细胞中表达。我们还对80%的酵母菌内吞缺陷基因敲除菌株进行了筛选，并鉴定出约200个内吞运输缺陷基因。

To elucidate the mechanisms how activated receptor is recruited to clathrin coated pits, we screened yeast genome library, and identified Bmh2p, yeast homologue of 14-3-3 protein, as a binding protein for yeast GPCR. In addition, we tagged 13 human GPCR with GFP and succeeded in expressing in yeast cells. We also performed screening of about 80 % of yeast gene knock out strains for endocytosis defect, and identified about 200 genes that exhibited defects in endocytic transport.