Study of plant cell polarity establishment by using advanced imaging and sequencing technology

为了阐明植物细胞极性建立的机制，我们进行了GNOM和VAN3的亚细胞定位分析、小泡运输调节剂的亚细胞定位分析和拟南芥突变体的遗传分析。而GNOM和VAN3在Pms处共定位，在细胞内表现出不同的定位。有趣的是，还观察到GNOM定位于非内体隔室。此外，通过下一代测序鉴定了一些可能与细胞极性建立有关的突变。

To elucidate the mechanism of plant cell polarity establishment, subcellular localization analysis of GNOM and VAN3, vesicle transport regulators, and genetic analysis using arabidopsis mutants were performed. Whereas GNOM and VAN3 colocalized at PMs, they showed differential localization intracellularly. Interestingly, GNOM localization at non-endosomal compartments was also observed. Additionally, some mutation that supposed to be involved in cell polarity establishment was identified by next generation sequencing.