Dynamics of microtubules and plasma membrane domain during secondary cell wall deposition

纤维素微纤维沉积的取向受皮层微管控制。本研究的目的是揭示皮层微管在次生细胞壁沉积过程中的动力学。我们产生了转基因白杨，其中微管被GFP表达标记。用GFP荧光技术观察了转基因植株叶片、根和韧皮部细胞的皮层微管。然而，由于GFP标记蛋白的低表达，在木质部纤维细胞中GFP荧光较弱。

Orientation of cellulose microfibril deposition is controlled by cortical microtubules. The aim of this study is to reveal dynamics of cortical microtubules during the secondary cell wall deposition. We generated transgenic aspens in which microtubules were labeled by GFP expression. Cortical microtubules were visualized by GFP fluorescence in leaf, root and phloem cells in the transgenic plants. However, GFP fluorescence was weak in xylem fiber cells due to low expression of GFP-tagged protein.