Effect of blastomere removal in the pre-implantation diagnosis on the epigenetic process and embryonic development

本研究的目的是阐明在胚胎发育过程中，从胚胎中剔除一个卵裂球是如何影响剩余卵裂球的分布的。我检查了一个有荧光珠标记的早期小鼠胚胎的卵裂球在胚胎发育中的运动。我们还培养和观察了向一个卵裂球注射珠粒的两细胞期胚胎。在几乎相同的发生率下，观察到这些小珠只定位于细胞内团或只定位于细胞滋养层。本研究表明，在胚胎基因组转录之前，被认为具有全能性的卵裂球有可能决定分化的命运。这一结果表明，我们需要进一步的研究来证实在早期胚胎中切除卵裂球的安全性。

The purpose of this study is to clarify how it affects the distribution of the remained blastomeres to eliminate one blastomere from embryo in embryonic development process. I examined the motion of one blastomere marked with fluorescent beads of the early mouse embryo in embryonic development.We also cultured and observed two-cell stage embryos which injected with beads to one blastomere. It was observed in the almost equal incidence rate that those beads are localized only in the inner cell mass or only in the cytotrophoblast cell layer. This study showed that there is a possibility that the fate of differentiation is determined to blastomeres that are considered to have a totipotency before embryonic genome transcription. This result indicates that we need additional investigation to confirm the safety of blastomere removal in the early embryo.