Effect of blastomere removal in the pre-implantation diagnosis on the epigenetic process and embryonic development

着床前诊断中卵裂球去除对表观遗传过程和胚胎发育的影响

• 批准号: 23890023
• 负责人: SATO Megumi
• 金额: $ 2.39万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-08-24 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890023/
• 关键词: 着床前診断; 割球細胞; 胚発育; 割球除去; 胚発育挙動

The purpose of this study is to clarify how it affects the distribution of the remained blastomeres to eliminate one blastomere from embryo in embryonic development process. I examined the motion of one blastomere marked with fluorescent beads of the early mouse embryo in embryonic development.We also cultured and observed two-cell stage embryos which injected with beads to one blastomere. It was observed in the almost equal incidence rate that those beads are localized only in the inner cell mass or only in the cytotrophoblast cell layer. This study showed that there is a possibility that the fate of differentiation is determined to blastomeres that are considered to have a totipotency before embryonic genome transcription. This result indicates that we need additional investigation to confirm the safety of blastomere removal in the early embryo.

本研究的目的是阐明在胚胎发育过程中从胚胎中消除一个卵裂球是如何影响剩余卵裂球的分布的。我检查了早期小鼠胚胎在胚胎发育过程中用荧光珠标记的一个卵裂球的运动情况。我们还培养并观察了在一个卵裂球中注射珠子的二细胞期胚胎。以几乎相同的发生率观察到，这些珠子仅定位于内细胞团或仅定位于细胞滋养层细胞层。这项研究表明，分化的命运有可能是由在胚胎基因组转录之前被认为具有全能性的卵裂球决定的。这一结果表明我们需要进一步的研究来确认早期胚胎中卵裂球去除的安全性。

项目成果

重症妊娠高血圧症候群を発症したEmery-Dreifuss型筋ジストロフィー合併妊娠の一例

妊娠并发Emery-Dreifuss肌营养不良症合并严重妊娠期高血压综合征1例

• 发表时间: 2012
• 作者: 佐藤恵

大規模災害時に広域母体搬送により母児ともに救命し得た骨髄異形成症候群合併妊娠の一例

一起患有骨髓增生异常综合征的孕妇在一次大规模灾难中通过广域产妇转运救活了母婴的案例。

• 发表时间: 2012
• 作者: Yamada A;Iwamoto T;Fukumoto E;Arakaki M;Miyamoto R;Sugawara Y;Komatsu H;Nakamura T;Fukumoto S;佐藤恵
• 通讯作者: 佐藤恵