Early diagnosis with in vivo laser confocal microscopy incytomegalovirus corneal endotheliitis and Acanthamoeba keratitis

在这项研究中，我们进行了体内激光共聚焦显微镜分析患者巨细胞病毒角膜内皮炎和阿米巴角膜炎。在巨细胞病毒角膜内皮炎中，使用获得的共聚焦图像创建了猫头鹰眼睛细胞的二维重建图;该图显示了圆形图案。此外，我们证明了猫头鹰的眼睛细胞共聚焦显微镜观察到的硬币状病变裂隙灯显微镜观察到之间的关联。在持续性阿米巴角膜炎的患者中，观察到特征性的囊肿侵入鲍曼层。这一发现表明，阿米巴囊肿侵入鲍曼氏层可能是一个有用的预测持续的临床过程。

In this study, we performed in vivo laser confocal microscopic analysis in patients with cytomegalovirus corneal endotheliitis and Acanthamoebakeratitis. In cytomegalovirus corneal endotheliitis, a two-dimensional reconstruction map of the owl’s eye cells was created using acquired confocal images; the maps showed circular patterns. Also, we demonstrated that there is an association between owl’s eye cells observed by confocal microscopy and coin-shaped lesions observed by slit-lampbiomicroscopy. In patients with persistence of Acanthamoeba keratitis, invasion of cysts into Bowman’s layer was characteristically observed. This finding suggests that invasion of Acanthamoeba cysts into Bowman’s layer may be a useful predictor for a persistent clinical course.