Cell therapy for radiation-induced osteonecrosis with non-cultured adipose derived cells

使用非培养脂肪来源的细胞治疗辐射引起的骨坏死

• 批准号: 23890159
• 负责人: KAWASAKI Takako
• 金额: $ 2.08万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890159/
• 关键词: 顎骨壊死; 細胞治療; 脂肪幹細胞; 放射線性顎骨壊死; 脂肪由来細胞; 非培養脂肪由来幹細胞; 放射線治療

Jaw-Osteonecrosis is a serious side effect for patients with head and neck cancer who received radiation and/or chemo-therapy. It causes not only the limitation on the ability of patients to tolerate cancer therapies in some cases but also the harmful effects on the quality of life. However, current clinical strategies cannot efficiently prevent the occurrence of jaw-osteonecrosis. Therefore, effective therapy should be developed urgently. Adipose derived cells (ADC) isolated from male C3H mice were transplanted to the female mice just after the irradiation through the tail vein. Adipose derived cells (ADC) have been isolated from adipose tissue by the simple method with collagenase digestion and centrifugation. After 8 and 12 weeks of irradiation (16Gy) with cisplatin (7mg/kg), the jaw bone was harvested, and the histological and immunological analyses were performed to clarify the effects of ADC transplantation. On the other hand, the direct implantation of freshly isolated ADC with minimal amount of BMP could induce the ectopic bone formation. After 12 weeks of irradiation, theADC-transplanted mice reduced the obliteration of cell and blood-vessels in bone tissue compared with irradiated/non-transplanted mice.

颌骨骨坏死是接受放射和/或化疗的头颈癌患者的严重副作用。它不仅在某些情况下限制了患者对癌症治疗的耐受能力，而且还对生活质量产生了有害影响。然而，目前的临床策略并不能有效地预防颌骨骨坏死的发生。因此，迫切需要开发有效的治疗方法。从雄性C3H小鼠分离脂肪来源细胞(ADC)，经尾静脉照射后立即移植到雌性小鼠体内。用胶原酶消化离心法从脂肪组织中分离出脂肪来源细胞(ADC)。用顺铂(7 mg/kg)照射8周和12周后，取颌骨，进行组织学和免疫学分析，以明确ADC移植的效果。而直接植入新鲜分离的ADC和微量的BMP可诱导异位成骨。照射12周后，与照射/未移植小鼠相比，移植ADC的小鼠骨组织中细胞和血管的闭塞程度减少。

项目成果

A case of voluminous tongue hemangioma treated with intralesional photocoagulation using a KTP laser

KTP激光病灶内光凝治疗舌体巨大血管瘤1例

• 发表时间: 2012
• 期刊: J Oral Maxillofac Surg, Med, and Pathol.
• 作者: Kawasaki T;Sumita Y;Tobita T;Ikeda M;Ohba S;Asahina I
• 通讯作者: Asahina I

In vivo comparison of the bone regeneration capability of human bone marrow concentrates vs. platelet-rich plasma.

• DOI: 10.1371/journal.pone.0040833
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhong W;Sumita Y;Ohba S;Kawasaki T;Nagai K;Ma G;Asahina I
• 通讯作者: Asahina I

A Review of toxicity superselective intra-arterial concurrent chemoradiotherapy (SIACC) for oral cancer

口腔癌超选择性动脉内同步放化疗（SIACC）的毒性评价

• 发表时间: 2011
• 期刊: Gan To Kagaku Ryoho.
• 作者: Shiraishi T;Uehara M;Ikeda M;Tajima N;Ikeda H;Kawasaki T;Asahina I
• 通讯作者: Asahina I

虚血環境モデルとしての重度低酸素培養条件がヒト歯根膜由来細胞の生存, 可塑性, 分化に与える影響について

严重缺氧培养条件作为缺血环境模型对人牙周膜来源细胞存活、可塑性和分化的影响

• 发表时间: 2012
• 作者: 川崎貴子;住田吉慶;縣秀樹;各務秀明;Tran SD;朝比奈泉
• 通讯作者: 朝比奈泉