Development of new Treatments for Osteoarthritis using Gene Transfer by Fullerene

利用富勒烯基因转移开发骨关节炎新疗法

• 批准号: 23890240
• 负责人: OHTANI Taishi
• 金额: $ 2.08万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890240/
• 关键词: 変形性関節症; 治療法; フラーレン; ナノカーボン; HIG-82; 局所投与; ウサギ; 滑膜細胞; 新規治療法; 抗原誘発関節炎モデル; 医療; 細胞; 組織; 滑膜炎細胞; 抗酸化作用; 抗炎症作用; 細胞為害性

Research for the purpose of a new treatment method using soluble fullerene to osteoarthritis was started. Soluble fullerene was added to the culture medium. Reaction of HIG-82 cells was observed. Trends suppression of cell proliferation was observed in the high density region. But it was not significant. Inflammatory cytokines in culture supernatants was measured by ELISA. However, the variation was not observed. These may be due to cytokine in the culture medium. It is necessary to consider the method of measurement. Only synovial inflammatory cells that confirm the anti-inflammatory effect of fullerene difficult have been found. Future comparison of inflammatory synovial cells and normal synovial cells is needed. In addition, it is expected to be added to the mechanical stress on normal synovial cells, on it, will continue to study the anti-inflammatory effect of the fullerene.

研究了一种利用可溶性富勒烯治疗骨关节炎的新方法。在培养基中加入可溶性富勒烯。观察HIG-82细胞的反应。高密度区有抑制细胞增殖的趋势。但这并不重要。ELISA法检测培养上清液中炎症因子的含量。然而，没有观察到这种变化。这可能与培养基中的细胞因子有关。有必要考虑测量方法。只有滑膜炎症细胞证实了富勒烯的抗炎作用。需要进一步比较炎性滑膜细胞和正常滑膜细胞。此外，可望将其加入到对正常滑膜细胞的机械应力中，对其继续研究富勒烯的抗炎作用。