Control of oxytalan fibers formation mechanism by Fibulin-5/EMILIN-1 complex

牙周膜中的氧化他兰纤维垂直排列，有助于牙周组织的功能维持。最近，我们用免疫荧光法证明，在拉伸条件下，奥克他兰纤维束聚集并变得粗大。然而，氧化他兰纤维和细胞主轴之间的确切关系尚不清楚。因此，在本研究中，我们研究了两者之间的关系。我们培养了人牙周膜成纤维细胞，并对细胞施加拉伸刺激(对照组：强度为0%，实验组：强度为5%)，并对氧化他兰纤维和细胞骨架肌动蛋白进行了免疫荧光检测。利用图像分析软件，对奥施他兰纤维与细胞长轴的平均夹角进行分析，结果显示，实验组奥施他兰纤维与细胞长轴的夹角接近矩形。这与角度分析测量的结果一致。这些结果表明，这个角度可能反映了体内细胞与氧化他兰纤维之间的关系的细胞外情况。

The oxytalan fibers in the periodontal ligament are arranged in vertically oriented and contribute to function maintenance of the periodontium. Recently, we demonstrated that, under stretc hing conditions, bundles of oxytalan fibers coalesce and become thick using immunofluorescence. However, a precise relation between oxytalan fibers and the major axis of the cells is unclear. Therefore, in the present study, we examind the relations between them.We cultured a human periodontal ligament fibroblasts and applied stretching stimulation to the cells (control group: strength 0% , experimental group: strength 5%) and performed immunofluorescence of the oxytalan fibers and cytoskeleton actin. Using image analysis software, we analyzed the average angle with the oxytalan fibers and a cell major axis.As for the experimental group, oxytalan fibers tend to be nearly rectanglular with the major axis of cells. It was consistent with that measured by the angle analysis. These results suggest that this angle may reflect the in vivo extracellar conditions about the relations between the cells and the oxytalan fibers.