Tests of the quantitative analytical method for airborne ice nucleating bacteria and its application for field experiments

本研究的目的是测试空气中冰核细菌的定量分析方法。通过实验室实验证实，经低温热处理后，紫丁香假单胞菌(Snowmax：人工引雪剂)的成冰活性降低。冰核细菌的这种热敏感性与其他冰核的敏感性不同。低温热处理可使新鲜雪中的冰核数量减少。这表明低温下的热敏感性可能是空气中冰核细菌定量检测的指标之一。

The objective of this study is to test an quantitative analytic method for airborne ice nucleating bacteria. By the laboratory experiments, it was confirmed that the ice nucleating activity of Pseudomonas syringae (Snowmax: artificial snow inducer) was decreased by the heat treatment at low temperature. This heat sensitivity of ice nucleating bacteria is different from the sensitivities of other ice nuclei. It was also observed that the numbers of ice nuclei in fresh snow were decreased by the heat treatment at low temperature. This suggests that the heat sensitivity at low temperature may be one of the indicator for the quantitative detection of airborne ice nucleating bacteria.